Rabbit PIBF1 Polyclonal Antibody | anti-PIBF1 antibody

PIBF1 Antibody

Cat. Num. AAA9607820

• Gene Names: PIBF1; PIBF; CEP90; JBTS33; C13orf24
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: PIBF1; Polyclonal Antibody; PIBF1 Antibody; C13orf24; KIAA1008; PIBF 1; PIBF; PIBF1_HUMAN; Progesterone immunomodulatory binding factor 1; Progesterone induced blocking factor 1; Progesterone-induced-blocking factor 1; RP11 505F3.1; anti-PIBF1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PIBF1 antibody detects endogenous levels of total PIBF1
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 757

• Applicable Applications for anti-PIBF1 antibody: Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:100; IF/ICC: 1:100-1:500
• Immunogen: A synthetic peptide of human PIBF1
• Subcellular Location: Cytoskeleton; Extracellular Region or Secreted; Nucleus; Cytoskeleton; Extracellular Region or Secreted; Extracellular Region or Secreted
• Tissue Specificity: Expressed at highest levels in testis. Moderate expression is detected in spleen, thymus, prostate, ovary, small intestine, and colon (PubMed:11935316). Expressed in the first trimester pregnancy decidua (PubMed:12516630). Localized to extravillous cytotrophoblast (at protein level). Also found in syncytiotrophoblast and part of the villous cytotrophoblast. Isoform 1 is expressed in first trimester and term villous trophoblast; trophoblast cells can additionally express other isoforms (PubMed:18817979). Overexpresed in solid tumors from stomach and uterus and in cells from ovary, cervical, breast, lymphoma and leukemia cancer (PubMed:25218441).
• Predicted Cross Reactivity: Horse, Sheep, Rabbit, Dog
• Similarity: Horse (100%), Sheep (90%), Rabbit (100%), Dog (100%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Immunohistochemistry (IHC)

(MBS9607820 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Western Blot (WB)

(Western blot analysis of extracts from hepg2, using PIBF1 Antibody. Lane 1 was treated with the blocking peptide.)

Immunofluorescence (IF)

(MBS9607820 staining LoVo by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Western Blot (WB)

(Western blot analysis of LoVo cell lysate, using PIBF1 Antibody. The lane on the left is treated with the antigen-specific peptide.)

Related Product Information for anti-PIBF1 antibody

Description: Progesterone-induced-blocking factor 1 is a protein that in humans is encoded by the PIBF1 gene. It has been shown to localize to the centrosome and has also been named CEP90. Mediator of progesterone that by acting on the phospholipase A2 enzyme interferes with arachidonic acid metabolism, induces a Th2 biased immune response, and by controlling NK activity exerts an anti-abortive effect.
Function: Isoform 1: Pericentriolar protein required to maintain mitotic spindle pole integrity (PubMed:21224392). Required for the centrosomal accumulation of PCM1 and the recruitment of centriolar satellite proteins such as BBS4. Via association with PCM1 may be involved in primary cilia formation (PubMed:23110211). Required for CEP63 centrosomal localization and its interaction with WDR62. Together with CEP63 promotes centriole duplication. Promotes the centrosomal localization of CDK2 (PubMed:26297806).
Subunit Structure: Isoform 1 interacts with PCM1, BBS4 and CEP63 (PubMed:21224392, PubMed:23110211, PubMed:26297806). Interacts with IL4R (PubMed:25218441).

NCBI and Uniprot Product Information

• NCBI GI #: 55769583
• NCBI GeneID: 10464
• NCBI Accession #: NP_006337.2
• NCBI GenBank Nucleotide #: NM_006346.3
• UniProt Accession #: Q8WXW3; O95664; Q6U9V2; Q6UG50; Q86V07; Q96SF4
• Molecular Weight: Observed: 90 kDa; Predicted: 90 kDa
• NCBI Official Full Name: progesterone-induced-blocking factor 1 isoform 2
• NCBI Official Synonym Full Names: progesterone immunomodulatory binding factor 1
• NCBI Official Symbol: PIBF1
• NCBI Official Synonym Symbols: PIBF; CEP90; JBTS33; C13orf24
• NCBI Protein Information: progesterone-induced-blocking factor 1
• UniProt Protein Name: Progesterone-induced-blocking factor 1
• Protein Family: Progesterone-induced-blocking factor
• UniProt Gene Name: PIBF1
• UniProt Synonym Gene Names: C13orf24; PIBF; PIBF; CEP90

NCBI Description

This gene encodes a protein that is induced by the steroid hormone progesterone and plays a role in the maintenance of pregnancy. The encoded protein regulates multiple facets of the immune system to promote normal pregnancy including cytokine synthesis, natural killer (NK) cell activity, and arachidonic acid metabolism. Low serum levels of this protein have been associated with spontaneous pre-term labor in humans. This protein may promote the proliferation, migration and invasion of glioma. [provided by RefSeq, Mar 2017]

Uniprot Description

Plays a role in ciliogenesis.