Human Progesterone-induced-blocking factor 1 ELISA Kit | PIBF1 elisa kit

Human Progesterone-induced-blocking factor 1 ELISA Kit

Cat. Num. AAA765415

• Gene Names: PIBF1; PIBF; CEP90; C13orf24
• Reactivity: Human
• Synonyms: Progesterone-induced-blocking factor 1; Human Progesterone-induced-blocking factor 1 ELISA Kit; PIBF1 (Progesterone-induced-blocking factor 1)/C13orf24/chromosome 13 open reading frame 24/KIAA1008/PIBF/progesterone immunomodulatory binding factor 1/progesterone-induced blocking factor 1/RP11-505F3.1; PIBF1 elisa kit

• Reactivity: Human
• Sequence Length: 757

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.78-50ng/ml
• Sensitivity: 0.469ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for PIBF1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-PIBF1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-PIBF1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the PIBF1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of PIBF1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 55769583
• NCBI GeneID: 10464
• NCBI Accession #: NP_006337.2
• NCBI GenBank Nucleotide #: NM_006346.2
• Molecular Weight: 35,071 Da
• NCBI Official Full Name: progesterone-induced-blocking factor 1
• NCBI Official Synonym Full Names: progesterone immunomodulatory binding factor 1
• NCBI Official Symbol: PIBF1
• NCBI Official Synonym Symbols: PIBF; CEP90; C13orf24
• NCBI Protein Information: progesterone-induced-blocking factor 1
• UniProt Protein Name: Progesterone-induced-blocking factor 1
• Protein Family: Progesterone-induced-blocking factor
• UniProt Gene Name: PIBF1
• UniProt Synonym Gene Names: C13orf24; PIBF; PIBF; CEP90
• UniProt Entry Name: PIBF1_HUMAN

Uniprot Description

PIBF1: Mediator of progesterone that by acting on the phospholipase A2 enzyme interferes with arachidonic acid metabolism, induces a Th2 biased immune response, and by controlling NK activity exerts an anti-abortive effect. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 13q22.1

Cellular Component: centrosome; extracellular space; nucleus

Molecular Function: interleukin-4 receptor binding; protein binding

Biological Process: immune system process; mitotic metaphase plate congression; negative regulation of interleukin-12 production; negative regulation of natural killer cell activation; negative regulation of prostaglandin biosynthetic process; negative regulation of tyrosine phosphorylation of Stat4 protein; positive regulation of interleukin-10 production; positive regulation of tyrosine phosphorylation of Stat6 protein; sensory cilium biogenesis