Western Blot (WB)
(Western Blot Positive WB detected in: Hela whole cell lysate, L02 whole cell lysate All lanes: PIBF1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 90, 26, 13, 36 kDa Observed band size: 90 kDa)
Rabbit anti-Human PIBF1 Polyclonal Antibody | anti-PIBF1 antibody
PIBF1 Antibody
Gene Names
PIBF1; PIBF; CEP90; C13orf24
Reactivity
Human
Applications
ELISA, Western Blot, Immunofluorescence
Purity
>95%, Protein G purified
Synonyms
PIBF1; Polyclonal Antibody; PIBF1 Antibody; Progesterone-induced-blocking factor 1 (PIBF) (Centrosomal protein of 90 kDa) (CEP90); C13orf24 PIBF; anti-PIBF1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH7.4
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH7.4
Applicable Applications for anti-PIBF1 antibody
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1:1000-1:5000
IF: 1:100-1:500
IF: 1:100-1:500
Conjugation
Non-conjugated
Immunogen
Recombinant Human Progesterone-induced-blocking factor 1 protein (1-757AA)
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
Western Blot (WB)
(Western Blot Positive WB detected in: Hela whole cell lysate, L02 whole cell lysate All lanes: PIBF1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 90, 26, 13, 36 kDa Observed band size: 90 kDa)
Western Blot (WB)
(Western Blot Positive WB detected in: Hela whole cell lysate, L02 whole cell lysate All lanes: PIBF1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 90, 26, 13, 36 kDa Observed band size: 90 kDa)
Immunofluorescence (IF)
(Immunofluorescence staining of Hela cells at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
Immunofluorescence (IF)
(Immunofluorescence staining of Hela cells at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
Related Product Information for anti-PIBF1 antibody
Isoform 1: Pericentriolar protein required to maintain mitotic spindle pole integrity (PubMed:21224392). Required for the centrosomal accumulation of PCM1 and the recruitment of centriolar satellite proteins such as BBS4. Via association with PCM1 may be involved in primary cilia formation (PubMed:23110211). Required for CEP63 centrosomal localization and its interaction with WDR62. Together with CEP63 promotes centriole duplication. Promotes the centrosomal localization of CDK2 (PubMed:26297806).
Product Categories/Family for anti-PIBF1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
89,805 Da
NCBI Official Full Name
progesterone-induced-blocking factor 1
NCBI Official Synonym Full Names
progesterone immunomodulatory binding factor 1
NCBI Official Symbol
PIBF1
NCBI Official Synonym Symbols
PIBF; CEP90; C13orf24
NCBI Protein Information
progesterone-induced-blocking factor 1; progesterone-induced blocking factor 1
UniProt Protein Name
Progesterone-induced-blocking factor 1
Protein Family
UniProt Gene Name
PIBF1
UniProt Synonym Gene Names
C13orf24; PIBF
UniProt Entry Name
PIBF1_HUMAN
Uniprot Description
PIBF1: Mediator of progesterone that by acting on the phospholipase A2 enzyme interferes with arachidonic acid metabolism, induces a Th2 biased immune response, and by controlling NK activity exerts an anti-abortive effect. 3 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 13q22.1
Cellular Component: centrosome
Molecular Function: protein binding
Research Articles on PIBF1
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Product Notes
The PIBF1 pibf1 (Catalog #AAA7136337) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PIBF1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PIBF1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF). WB: 1:1000-1:5000 IF: 1:100-1:500. Researchers should empirically determine the suitability of the PIBF1 pibf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIBF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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