Western Blot (WB)
(Western Blot: Sample: Mouse Small intestine lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse PLCb3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Mouse Phospholipase C Beta 3 Polyclonal Antibody | anti-PLCb3 antibody
Phospholipase C Beta 3, Phosphoinositide Specific (PLCb3) Polyclonal Antibody
Gene Names
Plcb3; mKIAA4098
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Phospholipase C Beta 3; Polyclonal Antibody; Phosphoinositide Specific (PLCb3) Polyclonal Antibody; 1-Phosphatidylinositol-4; 5-Bisphosphate Phosphodiesterase Beta-3; anti-PLCb3 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against PLCb3. It has been selected for its ability to recognize PLCb3 in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence Length
1234
Applicable Applications for anti-PLCb3 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Cross Reactivity
Mouse
Organism Species
Mouse
Fragment
PLCb3 (Asp318~Lys468)
Quality Control
Content: The quality control contains recombinant PLCb3 (Asp318~Lys468) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2057022
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Mouse Small intestine lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse PLCb3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Mouse Small intestine lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse PLCb3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
139,487 Da
NCBI Official Full Name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 isoform a
NCBI Official Synonym Full Names
phospholipase C, beta 3
NCBI Official Symbol
Plcb3
NCBI Official Synonym Symbols
mKIAA4098
NCBI Protein Information
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3
UniProt Protein Name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3
Protein Family
UniProt Gene Name
Plcb3
UniProt Synonym Gene Names
PLC-beta-3
Uniprot Description
PLCB3: a phosphatidylinositol-specific phospholipase C. Binds to calmodulin. The activated enzyme produces the second messenger molecules diacylglycerol and inositol 1,4,5-trisphosphate.
Protein type: Carbohydrate Metabolism - inositol phosphate; EC 3.1.4.11; Phospholipase
Chromosomal Location of Human Ortholog: 19 A|19 5.1 cM
Cellular Component: cytosol; nucleoplasm; nucleus; protein complex; sarcolemma
Molecular Function: cadherin binding; calcium ion binding; calmodulin binding; hydrolase activity; phosphoinositide phospholipase C activity; phosphoric diester hydrolase activity; signal transducer activity
Biological Process: G-protein coupled receptor signaling pathway; inositol trisphosphate metabolic process; lipid catabolic process; lipid metabolic process; phosphatidylinositol catabolic process; phospholipid catabolic process; post-Golgi vesicle-mediated transport; regulation of systemic arterial blood pressure; signal transduction
Research Articles on PLCb3
Similar Products
Product Notes
The PLCb3 plcb3 (Catalog #AAA2032520) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospholipase C Beta 3, Phosphoinositide Specific (PLCb3) Polyclonal Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Phospholipase C Beta 3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the PLCb3 plcb3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospholipase C Beta 3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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