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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Human G Protein Alpha Z Polyclonal Antibody | anti-GNaZ antibody

G Protein Alpha Z (GNaZ) Polyclonal Antibody

Gene Names
GNAZ; gz-alpha
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
G Protein Alpha Z; Polyclonal Antibody; G Protein Alpha Z (GNaZ) Polyclonal Antibody; GN-AZ; Guanine Nucleotide Binding Protein Alpha Z; G(x) alpha chain; anti-GNaZ antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against GNaZ. It has been selected for its ability to recognize GNaZ in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-GNaZ antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Cross Reactivity
Human
Organism Species
Human
Fragment
GNaZ (Gly2~Val219)
Quality Control
Content: The quality control contains recombinant GNaZ (Gly2~Val219) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2064128
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded brain tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin- embedded brain tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
40,924 Da
NCBI Official Full Name
guanine nucleotide-binding protein G(z) subunit alpha
NCBI Official Synonym Full Names
G protein subunit alpha z
NCBI Official Symbol
GNAZ
NCBI Official Synonym Symbols
gz-alpha
NCBI Protein Information
guanine nucleotide-binding protein G(z) subunit alpha
UniProt Protein Name
Guanine nucleotide-binding protein G(z) subunit alpha
UniProt Gene Name
GNAZ

NCBI Description

The protein encoded by this gene is a member of a G protein subfamily that mediates signal transduction in pertussis toxin-insensitive systms. This encoded protein may play a role in maintaining the ionic balance of perilymphatic and endolymphatic cochlear fluids. [provided by RefSeq, Jul 2008]

Uniprot Description

G-alpha(z): Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Belongs to the G-alpha family. G(i/o/t/z) subfamily.

Protein type: Endoplasmic reticulum; G protein, heterotrimeric; G protein, heterotrimeric alpha G((i/o/t/z)); Nuclear envelope

Chromosomal Location of Human Ortholog: 22q11.22-q11.23

Cellular Component: cell body; cytosol; dendrite; endoplasmic reticulum; extracellular exosome; heterotrimeric G-protein complex; nuclear envelope; plasma membrane

Molecular Function: G-protein beta/gamma-subunit complex binding; G-protein coupled receptor binding; GTP binding; GTPase activity; metabotropic serotonin receptor binding; metal ion binding; protein binding; receptor signaling protein activity

Biological Process: G-protein coupled receptor signaling pathway; G-protein signaling, adenylate cyclase inhibiting pathway; G-protein signaling, coupled to cAMP nucleotide second messenger; protein folding

Research Articles on GNaZ

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Product Notes

The GNaZ gnaz (Catalog #AAA2032187) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The G Protein Alpha Z (GNaZ) Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's G Protein Alpha Z can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the GNaZ gnaz for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "G Protein Alpha Z, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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