Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Human T-Cell Lymphoma Invasion And Metastasis Inducing Protein 1 Polyclonal Antibody | anti-TIAM1 antibody
T-Cell Lymphoma Invasion And Metastasis Inducing Protein 1 (TIAM1) Polyclonal Antibody
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a RAC1-specific guanine nucleotide exchange factor (GEF). GEFs mediate the exchange of guanosine diphosphate (GDP) for guanosine triphosphate (GTP). The binding of GTP induces a conformational change in RAC1 that allows downstream effectors to bind and transduce a signal. This gene thus regulates RAC1 signaling pathways that affect cell shape, migration, adhesion, growth, survival, and polarity, as well as influencing actin cytoskeletal formation, endocytosis, and membrane trafficking. This gene thus plays an important role in cell invasion, metastasis, and carcinogenesis. In addition to RAC1, the encoded protein activates additional Rho-like GTPases such as CDC42, RAC2, RAC3 and RHOA. This gene encodes multiple protein isoforms that experience a diverse array of intramolecular, protein-protein, and phosphorylation interactions as well as phosphoinositide binding. Both the longer and shorter isoforms have C-terminal Dbl homology (DH) and pleckstrin homology (PH) domains while only the longer isoforms of this gene have the N-terminal myristoylation site and the downstream N-terminal PH domain, ras-binding domain (RBD), and PSD-95/DlgA/ZO-1 (PDZ) domain. [provided by RefSeq, Jul 2017]
Uniprot Description
TIAM1: modulates the activity of RHO-like proteins and connects extracellular signals to cytoskeletal activities. Acts as a GDP- dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO-like GTPases and activates them. Activates RAC1, CDC42, and to a lesser extent RHOA. Regulates migration, cell-matrix and cell-cell adhesion by modulating the actin cytoskeleton through the GTPase, Rac1. Binding to Rac1 and the Arp2/3 complex may drive actin polymerization at specific sites, thus inducing dynamic morphological changes.
Protein type: GEF; GEF, Rac/Rho; Motility/polarity/chemotaxis; Oncoprotein
Chromosomal Location of Human Ortholog: 21q22.11
Cellular Component: axonal growth cone; cell soma; cell-cell contact zone; cytosol; dendritic spine; extrinsic component of cytoplasmic side of plasma membrane; intercellular junction; main axon; microtubule; nucleus; plasma membrane; postsynaptic density
Molecular Function: guanyl-nucleotide exchange factor activity; kinase binding; lipid binding; microtubule binding; protein binding; Rac guanyl-nucleotide exchange factor activity; receptor signaling protein activity; receptor tyrosine kinase binding; Rho guanyl-nucleotide exchange factor activity
Biological Process: apoptosis; cardiac muscle hypertrophy; cell migration; cell-matrix adhesion; ephrin receptor signaling pathway; G-protein coupled receptor signaling pathway; NMDA selective glutamate receptor signaling pathway; positive regulation of apoptosis; positive regulation of axonogenesis; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of epithelial to mesenchymal transition; positive regulation of JUN kinase activity; positive regulation of protein binding; positive regulation of Schwann cell chemotaxis; protein localization to membrane; Rac protein signal transduction; regulation of dopaminergic neuron differentiation; regulation of epithelial to mesenchymal transition; regulation of ERK1 and ERK2 cascade; regulation of modification of postsynaptic actin cytoskeleton; regulation of non-canonical Wnt signaling pathway; regulation of Rho protein signal transduction; regulation of small GTPase mediated signal transduction; response to cocaine; Wnt receptor signaling pathway, planar cell polarity pathway
Research Articles on TIAM1
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Product Notes
The TIAM1 tiam1 (Catalog #AAA2032162) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The T-Cell Lymphoma Invasion And Metastasis Inducing Protein 1 (TIAM1) Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's T-Cell Lymphoma Invasion And Metastasis Inducing Protein 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the TIAM1 tiam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "T-Cell Lymphoma Invasion And Metastasis Inducing Protein 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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