Western Blot (WB)
(Western Blot: Sample: Human HepG2 cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat PIK3AP1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Rat Phosphoinositide-3-Kinase Adaptor Protein 1 (PIK3AP1) Polyclonal Antibody | anti-PIK3AP1 antibody
Polyclonal Antibody to Phosphoinositide-3-Kinase Adaptor Protein 1 (PIK3AP1)
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Phosphoinositide-3-Kinase Adaptor Protein 1 (PIK3AP1); Polyclonal Antibody; Polyclonal Antibody to Phosphoinositide-3-Kinase Adaptor Protein 1 (PIK3AP1); anti-PIK3AP1 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against PIK3AP1. It has been selected for its ability to recognize PIK3AP1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- ETVD MLKSHIKEEL MQGEEADDVY ESMAHLSTDL LMKCSLNPGC DDELYESMAA FAPAATEDLY VEMLQASAGN PVSGESFSRP TKDSMIRKFL EGNSMKLASL EREQHPPHGE ELYHTVEEDE TFSVDLANRP PVPVPRPETS TPGPHPLPDN EPYISKVFAE KSQERVGNFY VSSESIRKEP LVRPWRDRPP SSIYDPFAGM KTPGQRQLIT LQEQVKLGIV NVDEAVLHFK EWQLNQKKRS ESFRFQQENL
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- ETVD MLKSHIKEEL MQGEEADDVY ESMAHLSTDL LMKCSLNPGC DDELYESMAA FAPAATEDLY VEMLQASAGN PVSGESFSRP TKDSMIRKFL EGNSMKLASL EREQHPPHGE ELYHTVEEDE TFSVDLANRP PVPVPRPETS TPGPHPLPDN EPYISKVFAE KSQERVGNFY VSSESIRKEP LVRPWRDRPP SSIYDPFAGM KTPGQRQLIT LQEQVKLGIV NVDEAVLHFK EWQLNQKKRS ESFRFQQENL
Sequence Length
805
Applicable Applications for anti-PIK3AP1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant PIK3AP1 (Glu397~Leu650) expressed in E.coli.
Cross Reactivity
Human, Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2081860
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Human HepG2 cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat PIK3AP1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Human HepG2 cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat PIK3AP1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Rat Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Rat Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
91,061 Da
NCBI Official Full Name
Phosphoinositide 3-kinase adapter protein 1
NCBI Official Symbol
Pik3ap1
NCBI Protein Information
phosphoinositide 3-kinase adapter protein 1
UniProt Protein Name
Pik3ap1 protein
Protein Family
UniProt Gene Name
Pik3ap1
Uniprot Description
BCAP: an adaptor protein for phosphoinositide-3-kinase. Plays a role in connecting B-cell antigen receptor signaling to phosphoinositide 3-kinase activation.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 10q24.1
Cellular Component: membrane; plasma membrane; cytosol
Molecular Function: identical protein binding
Biological Process: positive regulation of phosphoinositide 3-kinase cascade; regulation of inflammatory response; negative regulation of toll-like receptor signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor 7 signaling pathway; toll-like receptor 4 signaling pathway; positive regulation of toll-like receptor signaling pathway; toll-like receptor 2 signaling pathway
Research Articles on PIK3AP1
1. Observational study of gene-disease association and gene-environment interaction. (HuGE Navigator)
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Product Notes
The PIK3AP1 pik3ap1 (Catalog #AAA2006367) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Phosphoinositide-3-Kinase Adaptor Protein 1 (PIK3AP1) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Phosphoinositide-3-Kinase Adaptor Protein 1 (PIK3AP1) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the PIK3AP1 pik3ap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- ETVD MLKSHIKEEL MQGEEADDVY ESMAHLSTDL LMKCSLNPGC DDELYESMAA FAPAATEDLY VEMLQASAGN PVSGESFSRP TKDSMIRKFL EGNSMKLASL EREQHPPHGE ELYHTVEEDE TFSVDLANRP PVPVPRPETS TPGPHPLPDN EPYISKVFAE KSQERVGNFY VSSESIRKEP LVRPWRDRPP SSIYDPFAGM KTPGQRQLIT LQEQVKLGIV NVDEAVLHFK EWQLNQKKRS ESFRFQQENL. It is sometimes possible for the material contained within the vial of "Phosphoinositide-3-Kinase Adaptor Protein 1 (PIK3AP1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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