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Western Blot (WB)
(Western Blot: Sample: Recombinant PGP, Mouse.)
Rabbit anti-Mouse Phosphoglycolate Phosphatase Polyclonal Antibody | anti-PGP antibody
Phosphoglycolate Phosphatase (PGP) Polyclonal Antibody
Gene Names
Pgp; AUM; G3PP; AI481330; 1700012G19Rik
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Phosphoglycolate Phosphatase; Polyclonal Antibody; Phosphoglycolate Phosphatase (PGP) Polyclonal Antibody; anti-PGP antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against PGP. It has been selected for its ability to recognize PGP in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence Length
227
Applicable Applications for anti-PGP antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Cross Reactivity
Mouse
Organism Species
Mouse
Fragment
PGP (Leu81~Gly321)
Quality Control
Content: The quality control contains recombinant PGP (Leu81~Gly321) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2079760
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant PGP, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant PGP, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Large Intestine Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Large Intestine Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Pancreas Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Pancreas Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Liver Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Liver Tissue.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
34,541 Da
NCBI Official Full Name
glycerol-3-phosphate phosphatase
NCBI Official Synonym Full Names
phosphoglycolate phosphatase
NCBI Official Symbol
Pgp
NCBI Official Synonym Symbols
AUM; G3PP; AI481330; 1700012G19Rik
NCBI Protein Information
glycerol-3-phosphate phosphatase
UniProt Protein Name
Glycerol-3-phosphate phosphatase
Protein Family
UniProt Gene Name
Pgp
UniProt Synonym Gene Names
PGP
Uniprot Description
PGP: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family.
Protein type: Carbohydrate Metabolism - glyoxylate and dicarboxylate; EC 3.1.3.18; EC 3.1.3.48; Phosphatase (non-protein)
Chromosomal Location of Human Ortholog: 17|17 A3.3
Cellular Component: cytosol
Molecular Function: glycerol-1-phosphatase activity; glycerol-3-phosphatase activity; hydrolase activity; magnesium ion binding; metal ion binding; phosphoglycolate phosphatase activity; phosphoprotein phosphatase activity; phosphoric monoester hydrolase activity; protein tyrosine phosphatase activity
Biological Process: carbohydrate metabolic process; dephosphorylation; glycerol biosynthetic process; glycerophospholipid metabolic process; metabolic process; negative regulation of gluconeogenesis
Research Articles on PGP
Similar Products
Product Notes
The PGP pgp (Catalog #AAA2032390) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phosphoglycolate Phosphatase (PGP) Polyclonal Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Phosphoglycolate Phosphatase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the PGP pgp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phosphoglycolate Phosphatase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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