Rabbit anti-Human CDC25B Polyclonal Antibody | anti-CDC25B antibody

CDC25B (S353) (CDC25B, CDC25HU2, M-phase inducer phosphatase 2, Dual specificity phosphatase Cdc25B) (Biotin)

Cat. Num. AAA6283810

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CDC25B; Polyclonal Antibody; CDC25B (S353) (CDC25B; CDC25HU2; M-phase inducer phosphatase 2; Dual specificity phosphatase Cdc25B) (Biotin); Dual specificity phosphatase Cdc25B; anti-CDC25B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-CDC25B antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CDC25B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S353 of human CDC25B.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-CDC25B Antibody (S353) in Ramos cell line lysates (35ug/lane). CDC25B (arrow) was detected using the purified Pab.)

Related Product Information for anti-CDC25B antibody

CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined.

Product Categories/Family for anti-CDC25B antibody

Antibodies; Cell Division Cycle (CDC)

References

Uchida, S., et al., Biochem. Biophys. Res. Commun. 316(1):226-232 (2004). Ito, Y., et al., Int. J. Mol. Med. 13(3):431-435 (2004). Wu, W., et al., Cancer Res. 63(19):6195-6199 (2003). Mils, V., et al., Exp. Cell Res. 285(1):99-106 (2003). Theis-Febvre, N., et al., Oncogene 22(2):220-232 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 4757950
• NCBI GeneID: 994
• NCBI Accession #: NP_004349.1
• NCBI GenBank Nucleotide #: NM_004358.3
• UniProt Accession #: P30305; O43551; Q13971; Q5JX77; Q6RSS1; Q9BRA6; D3DVY1; D3DVY2; D3DVY3; D3DVY4
• Molecular Weight: 64,987 Da
• NCBI Official Full Name: M-phase inducer phosphatase 2 isoform 2
• NCBI Official Synonym Full Names: cell division cycle 25B
• NCBI Official Symbol: CDC25B
• NCBI Protein Information: M-phase inducer phosphatase 2; dual specificity phosphatase Cdc25B
• UniProt Protein Name: M-phase inducer phosphatase 2
• UniProt Gene Name: CDC25B
• UniProt Synonym Gene Names: CDC25HU2
• UniProt Entry Name: MPIP2_HUMAN

NCBI Description

CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq, Jul 2008]

Uniprot Description

CDC25B: a member of the MPI phosphatase family. Activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. Shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. At least four transcript variants for this gene exist. Three splice variant isoforms have been described.

Protein type: EC 3.1.3.48; Nuclear receptor co-regulator; Protein phosphatase, dual-specificity; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: nucleoplasm; spindle pole; centrosome; cytoplasm; cytosol

Molecular Function: protein binding; protein tyrosine phosphatase activity; protein kinase binding

Biological Process: positive regulation of cytokinesis; mitosis; cell division; positive regulation of protein kinase activity; positive regulation of cell proliferation; mitotic cell cycle; G2/M transition of mitotic cell cycle; positive regulation of mitotic cell cycle; protein amino acid phosphorylation; oocyte maturation; female meiosis I