Testing Data
(Gel: 8%SDS-PAGELane1: K562 cell lysateLane2: Jurkat cell lysateLane3: Hela cell lysateLane4: 293T cell lysateLysates: 40 ug per lanePrimary antibody: 1/550 dilutionSecondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilutionExposure time: 1 minute)
Rabbit anti-Human MCM3 Polyclonal Antibody | anti-MCM3 antibody
MCM3 Antibody
Western blotting: 1:500-1:2000
Immunohistochemistry: 1:50-1:200
Testing Data
(Gel: 8%SDS-PAGELane1: K562 cell lysateLane2: Jurkat cell lysateLane3: Hela cell lysateLane4: 293T cell lysateLysates: 40 ug per lanePrimary antibody: 1/550 dilutionSecondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilutionExposure time: 1 minute)
Testing Data
(Gel: 8%SDS-PAGELane1: K562 cell lysateLane2: Jurkat cell lysateLane3: Hela cell lysateLane4: 293T cell lysateLysates: 40 ug per lanePrimary antibody: 1/550 dilutionSecondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilutionExposure time: 1 minute)
Immunohistochemistry (IHC)
(The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using 31099 (MCM3 Antibody) at dilution 1/30, on the right is treated with the fusion protein.)
Immunohistochemistry (IHC)
(The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using 31099 (MCM3 Antibody) at dilution 1/30, on the right is treated with the fusion protein.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]
Uniprot Description
MCM3: a mini-chromosome maintenance protein, essential for the initiation of eukaryotic genome replication. Allows DNA to undergo a single round of replication per cell cycle. Required for DNA replication and cell proliferation.
Protein type: EC 3.6.4.12; DNA replication
Chromosomal Location of Human Ortholog: 6p12
Cellular Component: nucleoplasm; centrosome; alpha DNA polymerase:primase complex; MCM complex; intracellular membrane-bound organelle; membrane; perinuclear region of cytoplasm; nucleus
Molecular Function: protein binding; DNA helicase activity; DNA binding; ATP binding
Biological Process: DNA replication initiation; mitotic cell cycle; DNA strand elongation during DNA replication; DNA replication; DNA duplex unwinding; G1/S transition of mitotic cell cycle
Research Articles on MCM3
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Product Notes
The MCM3 mcm3 (Catalog #AAA9409864) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MCM3 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MCM3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). ELISA: 1:1000-1:5000 Western blotting: 1:500-1:2000 Immunohistochemistry: 1:50-1:200. Researchers should empirically determine the suitability of the MCM3 mcm3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MCM3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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