Western Blot (WB)
(Western Blot analysis of INTS12 expression in transfected 293T cell line by INTS12 polyclonal antibody. Lane 1: PHF22 transfected lysate (50.82kD). Lane 2: Non-transfected lysate.)
Mouse anti-Human PHF22 Polyclonal Antibody | anti-INTS12 antibody
PHF22 (Integrator Complex Subunit 12, PHD Finger Protein 22, INTS12, SBBI22)
Gene Names
INTS12; INT12; PHF22; SBBI22
Reactivity
Human
Applications
Western Blot, Immunofluorescence
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
PHF22; Polyclonal Antibody; PHF22 (Integrator Complex Subunit 12; PHD Finger Protein 22; INTS12; SBBI22); Anti -PHF22 (Integrator Complex Subunit 12; anti-INTS12 antibody
Host
Mouse
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human INTS12.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Sequence
MAATVNLELDPIFLKALGFLHSKSKDSAEKLKALLDESLARGIDSSYRPSQKDVEPPKISSTKNISIKQEPKISSSLPSGNNNGKVLTTEKVKKEAEKRPADKMKSDITEGVDIPKKPRLEKPETQSSPITVQSSKDLPMADLSSFEETSADDFAMEMGLACVVCRQMMVASGNQLVECQECHNLYHRDCHKPQVTDKEANDPRLVWYCARCTRQMKRMAQKTQKPPQKPAPAVVSVTPAVKDPLVKKPETKLKQETTFLAFKRTEVKTSTVISGNSSSASVSSSVTSGLTGWAAFAAKTSSAGPSTAKLSSTTQNNTGKPATSSANQKPVGLTGLATSSKGGIGSKIGSNNSTTPTVPLKPPPPLTLGKTGLSRSVSCDNVSKVGLPSPSSLVPGSSSQLSGNGNSGTSGPSGSTTSKTTSESSSSPSASLKGPTSQESQLNAMKRLQMVKKKAAQKKLKK
Applicable Applications for anti-INTS12 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
Suitable for use in Immunofluorescence and Western Blot.
Dilution: Immunofluorescence: 10ug/ml
Dilution: Immunofluorescence: 10ug/ml
Immunogen
Full length human INTS12, aa1-462 (NP_065128.2).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of INTS12 expression in transfected 293T cell line by INTS12 polyclonal antibody. Lane 1: PHF22 transfected lysate (50.82kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of INTS12 expression in transfected 293T cell line by INTS12 polyclonal antibody. Lane 1: PHF22 transfected lysate (50.82kD). Lane 2: Non-transfected lysate.)
Immunofluorescence (IF)
(Immunofluorescence of purified antibody to INTS12 on HepG2 cell. [antibody concentration 10ug/ml].)
Immunofluorescence (IF)
(Immunofluorescence of purified antibody to INTS12 on HepG2 cell. [antibody concentration 10ug/ml].)
Related Product Information for anti-INTS12 antibody
PHF22 is a component of the integrator complex stably associated with RNA polymerase II. The integrator complex mediates RNA polymerase-II-dependent transcription. This protein is recruited to the U1 and U2 snRNA genes and mediates the snRNA's 3' end processing.
Product Categories/Family for anti-INTS12 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,808 Da
NCBI Official Full Name
integrator complex subunit 12 isoform X3
NCBI Official Synonym Full Names
integrator complex subunit 12
NCBI Official Symbol
INTS12
NCBI Official Synonym Symbols
INT12; PHF22; SBBI22
NCBI Protein Information
integrator complex subunit 12; PHD finger protein 22; hypothetical nuclear factor SBBI22
UniProt Protein Name
Integrator complex subunit 12
Protein Family
UniProt Gene Name
INTS12
UniProt Synonym Gene Names
PHF22; Int12
UniProt Entry Name
INT12_HUMAN
NCBI Description
INTS12 is a subunit of the Integrator complex, which associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; MIM 180660) and mediates 3-prime end processing of small nuclear RNAs U1 (RNU1; MIM 180680) and U2 (RNU2; MIM 180690) (Baillat et al., 2005 [PubMed 16239144]).[supplied by OMIM, Mar 2008]
Uniprot Description
PHF22: Component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes.
Protein type: RNA-binding
Chromosomal Location of Human Ortholog: 4q24
Cellular Component: integrator complex
Molecular Function: protein binding; zinc ion binding
Biological Process: snRNA processing
Research Articles on INTS12
Similar Products
Product Notes
The INTS12 ints12 (Catalog #AAA6013446) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PHF22 (Integrator Complex Subunit 12, PHD Finger Protein 22, INTS12, SBBI22) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PHF22 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). Suitable for use in Immunofluorescence and Western Blot. Dilution: Immunofluorescence: 10ug/ml. Researchers should empirically determine the suitability of the INTS12 ints12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MAATVNLELD PIFLKALGFL HSKSKDSAEK LKALLDESLA RGIDSSYRPS QKDVEPPKIS STKNISIKQE PKISSSLPSG NNNGKVLTTE KVKKEAEKRP ADKMKSDITE GVDIPKKPRL EKPETQSSPI TVQSSKDLPM ADLSSFEETS ADDFAMEMGL ACVVCRQMMV ASGNQLVECQ ECHNLYHRDC HKPQVTDKEA NDPRLVWYCA RCTRQMKRMA QKTQKPPQKP APAVVSVTPA VKDPLVKKPE TKLKQETTFL AFKRTEVKTS TVISGNSSSA SVSSSVTSGL TGWAAFAAKT SSAGPSTAKL SSTTQNNTGK PATSSANQKP VGLTGLATSS KGGIGSKIGS NNSTTPTVPL KPPPPLTLGK TGLSRSVSCD NVSKVGLPSP SSLVPGSSSQ LSGNGNSGTS GPSGSTTSKT TSESSSSPSA SLKGPTSQES QLNAMKRLQM VKKKAAQKKL KK. It is sometimes possible for the material contained within the vial of "PHF22, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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