Immunofluorescence (IF)
(Fluorescent image of HUVEC cell stained with HOXD8 Antibody (C-term). HUVEC cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HOXD8 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).HOXD8 immunoreactivity is localized to Nucleus significantly.)
Rabbit anti-Human HOXD8 Polyclonal Antibody | anti-HOXD8 antibody
HOXD8 Antibody (C-term)
WB~~1:1000
Immunofluorescence (IF)
(Fluorescent image of HUVEC cell stained with HOXD8 Antibody (C-term). HUVEC cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HOXD8 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).HOXD8 immunoreactivity is localized to Nucleus significantly.)
Immunofluorescence (IF)
(Fluorescent image of HUVEC cell stained with HOXD8 Antibody (C-term). HUVEC cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HOXD8 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).HOXD8 immunoreactivity is localized to Nucleus significantly.)
Western Blot (WB)
(HOXD8 Antibody (C-term) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the HOXD8 antibody detected the HOXD8 protein (arrow).)
Western Blot (WB)
(HOXD8 Antibody (C-term) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the HOXD8 antibody detected the HOXD8 protein (arrow).)
homeobox genes encode a highly conserved family of transcription
factors that play an important role in morphogenesis in all
multicellular organisms. Mammals possess four similar homeobox gene
clusters, HOXA, HOXB, HOXC and HOXD, located on different
chromosomes, consisting of 9 to 11 genes arranged in tandem. This
gene is one of several homeobox HOXD genes located in a cluster on
chromosome 2. Deletions that remove the entire HOXD gene cluster or
the 5' end of this cluster have been associated with severe limb
and genital abnormalities. In addition to effects during
embryogenesis, this particular gene may also play a role in adult
urogenital tract function.
Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009)
Harada, K., et al. J. Cell. Sci. 122 (PT 21), 3923-3930 (2009) :
Goodman, F.R. Am. J. Med. Genet. 112(3):256-265(2002)
Kosaki, K., et al. Teratology 65(2):50-62(2002)
NCBI and Uniprot Product Information
NCBI Description
This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, located on different chromosomes, consisting of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXD genes located in a cluster on chromosome 2. Deletions that remove the entire HOXD gene cluster or the 5' end of this cluster have been associated with severe limb and genital abnormalities. In addition to effects during embryogenesis, this particular gene may also play a role in adult urogenital tract function. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Dec 2010]
Uniprot Description
HOXD8: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Belongs to the Antp homeobox family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 2q31.1
Cellular Component: nucleus
Biological Process: transcription, DNA-dependent; skeletal morphogenesis; determination of anterior/posterior axis, embryo; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter
Research Articles on HOXD8
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Product Notes
The HOXD8 hoxd8 (Catalog #AAA9209494) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 243-270. The HOXD8 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HOXD8 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), ELISA (EIA), Western Blot (WB). IF~~1:10~50 WB~~1:1000. Researchers should empirically determine the suitability of the HOXD8 hoxd8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HOXD8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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