Mouse anti-Human CD25 Antibody | anti-CD25 antibody

Anti-Hu CD25 Biotin

Cat. Num. AAA1801089

• Gene Names: IL2RA; p55; CD25; IL2R; IMD41; TCGFR; IDDM10
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
• Synonyms: CD25; Anti-Hu CD25 Biotin; interleukin 2 receptor subunit alpha; IL-2R α chain; Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2R subunit alpha; IL-2-RA; IL2-RA; p55; TAC antigen; IDDM10; TCGFR; anti-CD25 antibody

• Host: Mouse
• Reactivity: Human
• Isotype: IgG1
• Clone Number: MEM-181
• Specificity: The antibody MEM-181 reacts with an extracellular epitope of CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.
• Purity/Purification: The purified antibody is conjugated with biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
• Form/Format: Phosphate buffered saline (PBS) solution with 15 mM sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 272

• Applicable Applications for anti-CD25 antibody: Flow Cytometry (FC/FACS)
• Application Notes: FC/FACS: QC tested
• Immunogen: PHA-activated peripheral blood leucocytes
• Conjugation: Biotin
• Preparation and Storage: Store at 2-8 degree C.

Flow Cytometry (FC/FACS)

(Flow cytometry analysis (surface staining) of PHA-stimulated (3 days) human PBMC with anti-CD25 (MEM-181) biotin, streptavidin-APC.)

Related Product Information for anti-CD25 antibody

CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. Humanized anti CD25 antibodies represent a useful tool to reduce the incidence of allograft rejection as well as the severity of graft versus host reaction, and radioimmunoconjugates of anti-CD25 antibodies can be used against CD25 expressing lymphomas.

NCBI and Uniprot Product Information

• NCBI GI #: 4557667
• NCBI GeneID: 3559
• NCBI Accession #: NP_000408.1
• NCBI GenBank Nucleotide #: NP_000408.1
• UniProt Accession #: P01589
• NCBI Official Full Name: interleukin-2 receptor subunit alpha isoform 1
• NCBI Official Synonym Full Names: interleukin 2 receptor subunit alpha
• NCBI Official Symbol: IL2RA
• NCBI Official Synonym Symbols: p55; CD25; IL2R; IMD41; TCGFR; IDDM10
• NCBI Protein Information: interleukin-2 receptor subunit alpha
• UniProt Protein Name: Interleukin-2 receptor subunit alpha
• UniProt Gene Name: IL2RA
• UniProt Synonym Gene Names: IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA
• UniProt Entry Name: IL2RA_HUMAN

NCBI Description

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]

Uniprot Description

IL2RA: Receptor for interleukin-2. Non-covalent dimer of an alpha and a beta subunit. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 10p15-p14

Cellular Component: integral to membrane; plasma membrane; external side of plasma membrane

Molecular Function: interleukin-2 receptor activity; drug binding; interleukin-2 binding

Biological Process: Notch signaling pathway; regulation of T cell homeostatic proliferation; apoptosis; negative regulation of immune response; negative regulation of defense response to virus; positive regulation of activated T cell proliferation; cell proliferation; negative regulation of T cell proliferation; inflammatory response to antigenic stimulus; cell surface receptor linked signal transduction; positive regulation of T cell differentiation; negative regulation of inflammatory response; immune response; inflammatory response; activated T cell apoptosis

Disease: Interleukin 2 Receptor, Alpha, Deficiency Of; Diabetes Mellitus, Insulin-dependent, 10