Western Blot (WB)
(Anti-PHF1 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: MCF7 Whole Cell LysateLane C: NCI-H1299 Whole Cell LysateLane D: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:62 kDaObserved band size:67 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Human PHF1 Polyclonal Antibody | anti-PHF1 antibody
Anti-PHF1 Antibody, Rabbit Polyclonal
ICC/IF: 1:1500-1:50,000
Western Blot (WB)
(Anti-PHF1 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: MCF7 Whole Cell LysateLane C: NCI-H1299 Whole Cell LysateLane D: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:62 kDaObserved band size:67 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-PHF1 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: MCF7 Whole Cell LysateLane C: NCI-H1299 Whole Cell LysateLane D: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:62 kDaObserved band size:67 kDa(We are unsure as to the identity of these extra bands.))
Immunofluorescence (IF)
(Immunofluorescence staining of PHF1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human PHF1 polyclonal antibody (1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to nucleus.)
Immunofluorescence (IF)
(Immunofluorescence staining of PHF1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human PHF1 polyclonal antibody (1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to nucleus.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a Polycomb group protein. The protein is a component of a histone H3 lysine-27 (H3K27)-specific methyltransferase complex, and functions in transcriptional repression of homeotic genes. The protein is also recruited to double-strand breaks, and reduced protein levels results in X-ray sensitivity and increased homologous recombination. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
Uniprot Description
PHF1: Transcriptional repressor. May promote methylation of histone H3 on 'Lys-27' by the PRC2/EED-EZH2 complex. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription factor
Chromosomal Location of Human Ortholog: 6p21.3
Cellular Component: nucleoplasm; ESC/E(Z) complex; cytoplasm; microtubule organizing center; nucleus
Molecular Function: zinc ion binding; transcription factor activity; methylated histone residue binding
Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; negative regulation of gene expression, epigenetic; gene expression; chromatin modification; response to DNA damage stimulus; regulation of gene expression, epigenetic
Research Articles on PHF1
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Product Notes
The PHF1 phf1 (Catalog #AAA8101463) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PHF1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PHF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). WB: 1:500-1:1000 ICC/IF: 1:1500-1:50,000. Researchers should empirically determine the suitability of the PHF1 phf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PHF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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