Western Blot (WB)
(Western Blot: Western blot analysis of human PHD2, using MBS611010. Samples: Recombinant DYKDDDDK-His-PHD1, PHD2 and PHD3 (10ng/lane), HeLa whole cell lysate and MEFs.)
Rabbit anti-Human PHD2 Polyclonal Antibody | anti-LOC100170695 antibody
PHD2 (Prolyl Hydroxylase Domain Containing Protein 2, PHD-2, Chromosome 1 Open Reading Frame 12, C1orf12, DKFZp761F179, ECYT3, EGL-9 Homolog of C. elegans 1, EGL Nine (C. elegans) Homolog 1, Egl Nine Homolog 1, EGLN1, HIF Prolyl Hydroxylase 2, HIF-PH2, HI
Reactivity
Human
Applications
Western Blot
Purity
Affinity PurifiedPurified by peptide immunoaffinity chromatography.
Synonyms
PHD2; Polyclonal Antibody; PHD2 (Prolyl Hydroxylase Domain Containing Protein 2; PHD-2; Chromosome 1 Open Reading Frame 12; C1orf12; DKFZp761F179; ECYT3; EGL-9 Homolog of C. elegans 1; EGL Nine (C. elegans) Homolog 1; Egl Nine Homolog 1; EGLN1; HIF Prolyl Hydroxylase 2; HIF-PH2; HI; Anti -PHD2 (Prolyl Hydroxylase Domain Containing Protein 2; anti-LOC100170695 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
This antibody is specific for human HIF prolyl hydroxylase 2. There is 83% homology between the immunogen used for this antibody and the rat and mouse proteins. Species Crossreactivity: Human. Does not appear to work in mouse. Other species have not been tested.
Purity/Purification
Affinity Purified
Purified by peptide immunoaffinity chromatography.
Purified by peptide immunoaffinity chromatography.
Applicable Applications for anti-LOC100170695 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Immunogen
A synthetic peptide made to a C-terminal fragment of the human protein sequence of HIF prolyl hydroxylase 2 (residues 350-426). [LocusLink ID 54583].
Positive Control
Human g;ioblastoma cells
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western Blot: Western blot analysis of human PHD2, using MBS611010. Samples: Recombinant DYKDDDDK-His-PHD1, PHD2 and PHD3 (10ng/lane), HeLa whole cell lysate and MEFs.)
Western Blot (WB)
(Western Blot: Western blot analysis of human PHD2, using MBS611010. Samples: Recombinant DYKDDDDK-His-PHD1, PHD2 and PHD3 (10ng/lane), HeLa whole cell lysate and MEFs.)
Flow Cytometry (FC/FACS)
(Flow Cytometry: MBS611010- Flow cytometric detection of PHD2, 10e6 Jurkat cells were fixed, permeabilized, and stained with 3.0 ug/ml MBS611010 in a 150ul reaction.)
Flow Cytometry (FC/FACS)
(Flow Cytometry: MBS611010- Flow cytometric detection of PHD2, 10e6 Jurkat cells were fixed, permeabilized, and stained with 3.0 ug/ml MBS611010 in a 150ul reaction.)
Related Product Information for anti-LOC100170695 antibody
HIF prolyl hydroxylase 2 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, HPHs convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis.
Product Categories/Family for anti-LOC100170695 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The LOC100170695 (Catalog #AAA611010) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PHD2 (Prolyl Hydroxylase Domain Containing Protein 2, PHD-2, Chromosome 1 Open Reading Frame 12, C1orf12, DKFZp761F179, ECYT3, EGL-9 Homolog of C. elegans 1, EGL Nine (C. elegans) Homolog 1, Egl Nine Homolog 1, EGLN1, HIF Prolyl Hydroxylase 2, HIF-PH2, HI reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PHD2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Researchers should empirically determine the suitability of the LOC100170695 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PHD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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