Rabbit anti-Human, Mouse PGD Polyclonal Antibody | anti-PGD antibody

PGD, ID (PGD, PGDH, 6-phosphogluconate dehydrogenase, decarboxylating) (FITC)

Cat. Num. AAA6333016

• Gene Names: PGD; 6PGD
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PGD; Polyclonal Antibody; ID (PGD; PGDH; 6-phosphogluconate dehydrogenase; decarboxylating) (FITC); decarboxylating; anti-PGD antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-PGD antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PGD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 242-271 amino acids from the Central region of human PGD.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PGD Antibody (Center) western blot analysis in 293,MCF-7 cell line lysates (35ug/lane).This demonstrates the PGD antibody detected PGD protein (arrow).)

Western Blot (WB)

(PGD Antibody (Center) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the PGD antibody detected PGD protein (arrow).)

Immunohistochemistry (IHC)

(PGD Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PGD Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(PGD Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-PGD antibody

6-phosphogluconate dehydrogenase is the second dehydrogenase in the pentose phosphate shunt. Deficiency of this enzyme is generally asymptomatic, and the inheritance of this disorder is autosomal dominant. Hemolysis results from combined deficiency of 6-phosphogluconate dehydrogenase and 6-phosphogluconolactonase suggesting a synergism of the two enzymopathies.

Product Categories/Family for anti-PGD antibody

Antibodies; Enzymes; Dehydrogenase

References

Lehner, B., et al. Genome Res. 14(7):1315-1323(2004). Kerimov, B.F. Vopr. Med. Khim. 48(5):490-496(2002). Rippa, M., et al. Biochim. Biophys. Acta 1429(1):83-92(1998).

NCBI and Uniprot Product Information

• NCBI GI #: 40068518
• NCBI GeneID: 5226
• NCBI Accession #: NP_002622.2
• NCBI GenBank Nucleotide #: NM_002631.2
• UniProt Accession #: P52209; Q9BWD8; A8K2Y9
• Molecular Weight: 53,140 Da
• NCBI Official Full Name: 6-phosphogluconate dehydrogenase, decarboxylating
• NCBI Official Synonym Full Names: phosphogluconate dehydrogenase
• NCBI Official Symbol: PGD
• NCBI Official Synonym Symbols: 6PGD
• NCBI Protein Information: 6-phosphogluconate dehydrogenase, decarboxylating
• UniProt Protein Name: 6-phosphogluconate dehydrogenase, decarboxylating
• UniProt Gene Name: PGD
• UniProt Synonym Gene Names: PGDH
• UniProt Entry Name: 6PGD_HUMAN

NCBI Description

6-phosphogluconate dehydrogenase is the second dehydrogenase in the pentose phosphate shunt. Deficiency of this enzyme is generally asymptomatic, and the inheritance of this disorder is autosomal dominant. Hemolysis results from combined deficiency of 6-phosphogluconate dehydrogenase and 6-phosphogluconolactonase suggesting a synergism of the two enzymopathies. [provided by RefSeq, Jul 2008]

Uniprot Description

PGD: an oxidative carboxylase that catalyses the decarboxylating reduction of 6-phosphogluconate into ribulose 5-phosphate in the presence of NADP. This reaction is a component of the hexose mono-phosphate shunt and pentose phosphate pathways.

Protein type: Oxidoreductase; Carbohydrate Metabolism - pentose phosphate pathway; Other Amino Acids Metabolism - glutathione; EC 1.1.1.44

Chromosomal Location of Human Ortholog: 1p36.22

Cellular Component: nucleus; cytosol

Molecular Function: phosphogluconate dehydrogenase (decarboxylating) activity; NADP binding

Biological Process: pentose-phosphate shunt; pentose-phosphate shunt, oxidative branch; carbohydrate metabolic process; pathogenesis; D-gluconate metabolic process; pentose biosynthetic process