Canine Glutathione peroxidase ELISA Kit | GSH-Px elisa kit

Canine Glutathione peroxidase ELISA Kit

Cat. Num. AAA736920

• Gene Names: GPX3; GPx-P; GSHPx-3; GSHPx-P
• Reactivity: Canine
• Synonyms: Glutathione peroxidase; Canine Glutathione peroxidase ELISA Kit; GSH-Px elisa kit

• Reactivity: Canine

• Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GSH-Px elisa kit

Intended Uses: This GPX ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine GPX. This ELISA kit for research use only!

Principle of the Assay: GPX ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-GPX antibody and an GPX-HRP conjugate. The assay sample and buffer are incubated together with GPX-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GPX concentration since GPX from samples and GPX-HRP conjugate compete for the anti-GPX antibody binding site. Since the number of sites is limited, as more sites are occupied by GPX from the sample, fewer sites are left to bind GPX-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GPX concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 312004115
• NCBI GeneID: 2878
• UniProt Accession #: P22352; O43787; Q86W78; Q9NZ74; Q9UEL1
• Molecular Weight: 25,552 Da
• NCBI Official Full Name: glutathione peroxidase
• NCBI Official Synonym Full Names: glutathione peroxidase 3 (plasma)
• NCBI Official Symbol: GPX3
• NCBI Official Synonym Symbols: GPx-P; GSHPx-3; GSHPx-P
• NCBI Protein Information: glutathione peroxidase 3; GPx-3; plasma glutathione peroxidase; extracellular glutathione peroxidase
• UniProt Protein Name: Glutathione peroxidase 3
• Protein Family: Glutathione peroxidase
• UniProt Gene Name: GPX3
• UniProt Synonym Gene Names: GPXP; GPx-3; GSHPx-3; GPx-P; GSHPx-P
• UniProt Entry Name: GPX3_HUMAN

NCBI Description

This gene product belongs to the glutathione peroxidase family, which functions in the detoxification of hydrogen peroxide. It contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jul 2008]

Uniprot Description

GPX3: Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. Belongs to the glutathione peroxidase family.

Protein type: Secreted, signal peptide; Secreted; EC 1.11.1.9; Oxidoreductase; Other Amino Acids Metabolism - glutathione; Lipid Metabolism - arachidonic acid

Chromosomal Location of Human Ortholog: 5q33.1

Cellular Component: extracellular space; extracellular region

Molecular Function: selenium binding; glutathione peroxidase activity; transcription factor binding

Biological Process: response to reactive oxygen species; response to lipid hydroperoxide; hydrogen peroxide catabolic process; protein homotetramerization