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Western Blot (WB) (All lanes : Anti-PGAP1 Antibody (N-Term) at 1:2000 dilutionLane 1: SH-SY5Y whole cell lysateLane 2: Hela whole cell lysateLane 3: 293 whole cell lysateLane 4: mouse kidney lysateLane 5: mouse testis lysateLane 6: rat kidney lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 105 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit PGAP1 Polyclonal Antibody | anti-PGAP1 antibody

PGAP1 Antibody (N-Term)

Gene Names
PGAP1; Bst1; MRT42; SPG67; ISPD3024
Reactivity
Human, Mouse, Rat
Predicted: Mouse, Rat
Applications
Western Blot, ELISA
Synonyms
PGAP1; Polyclonal Antibody; PGAP1 Antibody (N-Term); GPI inositol-deacylase; 3.1.-.-; Post-GPI attachment to proteins factor 1; hPGAP1; anti-PGAP1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Mouse, Rat
Clonality
Polyclonal
Isotype
Ig
Specificity
This PGAP1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 90-122 amino acids from human PGAP1.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
748
Applicable Applications for anti-PGAP1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:2000
Antigen Type
Synthesized Peptide
Antigen Source
Human
Cellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-PGAP1 Antibody (N-Term) at 1:2000 dilutionLane 1: SH-SY5Y whole cell lysateLane 2: Hela whole cell lysateLane 3: 293 whole cell lysateLane 4: mouse kidney lysateLane 5: mouse testis lysateLane 6: rat kidney lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 105 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (All lanes : Anti-PGAP1 Antibody (N-Term) at 1:2000 dilutionLane 1: SH-SY5Y whole cell lysateLane 2: Hela whole cell lysateLane 3: 293 whole cell lysateLane 4: mouse kidney lysateLane 5: mouse testis lysateLane 6: rat kidney lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 105 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-PGAP1 antibody
Involved in inositol deacylation of GPI-anchored proteins. GPI inositol deacylation may important for efficient transport of GPI-anchored proteins from the endoplasmic reticulum to the Golgi (By similarity).
Product Categories/Family for anti-PGAP1 antibody
References
Tanaka S.,et al.J. Biol. Chem. 279:14256-14263 (2004). Ota T.,et al.Nat. Genet. 36:40-45 (2004). Bechtel S.,et al.BMC Genomics 8:399-399 (2007). Hillier L.W.,et al.Nature 434:724-731 (2005). Clark H.F.,et al.Genome Res. 13:2265-2270 (2003).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
105383 Da
NCBI Official Full Name
GPI inositol-deacylase isoform 2
NCBI Official Synonym Full Names
post-GPI attachment to proteins 1
NCBI Official Symbol
PGAP1
NCBI Official Synonym Symbols
Bst1; MRT42; SPG67; ISPD3024
NCBI Protein Information
GPI inositol-deacylase
UniProt Protein Name
GPI inositol-deacylase
UniProt Gene Name
PGAP1
UniProt Synonym Gene Names
hPGAP1

NCBI Description

The protein encoded by this gene functions early in the glycosylphosphatidylinositol (GPI) biosynthetic pathway, catalyzing the inositol deacylation of GPI. The encoded protein is required for the production of GPI that can attach to proteins, and this may be an important factor in the transport of GPI-anchored proteins from the endoplasmic reticulum to the Golgi. Defects in this gene are a cause of mental retardation, autosomal recessive 42. [provided by RefSeq, Mar 2016]

Uniprot Description

PGAP1: Involved in inositol deacylation of GPI-anchored proteins. GPI inositol deacylation may important for efficient transport of GPI-anchored proteins from the endoplasmic reticulum to the Golgi. Belongs to the GPI inositol-deacylase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.-.-; Glycan Metabolism - glycosylphosphatidylinositol (GPI)-anchor biosynthesis; Hydrolase; Lipase; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 2q33.1

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane

Molecular Function: hydrolase activity, acting on ester bonds; nuclease activity; phosphoric ester hydrolase activity

Biological Process: attachment of GPI anchor to protein; myo-inositol transport

Disease: Mental Retardation, Autosomal Recessive 42

Research Articles on PGAP1

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Product Notes

The PGAP1 pgap1 (Catalog #AAA9231013) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PGAP1 Antibody (N-Term) reacts with Human, Mouse, Rat Predicted: Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PGAP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:2000. Researchers should empirically determine the suitability of the PGAP1 pgap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PGAP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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