Mouse anti-Human PEPCK2 Monoclonal Antibody | anti-PEPCK2 antibody

PEPCK2 (Phosphoenolpyruvate Carboxykinase [GTP], Mitochondrial, PEPCK-M, Phosphoenolpyruvate Carboxylase, PCK2) (Biotin)

Cat. Num. AAA6143482

• Gene Names: PCK2; PEPCK; PEPCK2; PEPCK-M
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PEPCK2; Monoclonal Antibody; PEPCK2 (Phosphoenolpyruvate Carboxykinase [GTP]; Mitochondrial; PEPCK-M; Phosphoenolpyruvate Carboxylase; PCK2) (Biotin); anti-PEPCK2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 2C9
• Specificity: Recognizes human PCK2.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-PEPCK2 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-641 from human PCK2 (AAH01454) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MAALYRPGLRLNWHGLSPLGWPSCRSIQTLRVLSGDLGQLPTGIRDFVEHSARLCQPEGIHICDGTEAENTATLTLLEQQGLIRKLPKYNNCWLARTDPKDVARVESKTVIVTPSQRDTVPLPPGGARGQLGNWMSPADFQRAVDERFPGCMQGRTMYVLPFSMGPVGSPLSRIGVQLTDSAYVVASMRIMTRLGTPVLQALGDGDFVKCLHSVGQPLTGQGEPVSQWPCNPEKTLIGHVPDQREIISFGSGYGGNSLLGKKCFALRIASRLARDEGWLAEHMLILGITSPAGKKRYVAAAFPSACGKTNLAMMRPALPGWKVECVGDDIAWMRFDSEGRLRAINPENGFFGVAPGTSATTNPNAMATIQSNTIFTNVAETSDGGVYWEGIDQPLPPGVTVTSWLGKPWKPGDKEPCAHPNSRFCAPARQCPIMDPAWEAPEGVPIDAIIFGGRRPKGVPLVYEAFNWRHGVFVGSAMRSESTAAAEHKGKIIMHDPFAMRPFFGYNFGHYLEHWLSMEGRKGAQLPRIFHVNWFRRDEAGHFLWPGFGENARVLDWICRRLEGEDSARETPIGLVPKEGALDLSGLRAIDTTQLFSLPKDFWEQEVRDIRSYLTEQVNQDLPKEVLAELEALERRVHKM
• Conjugate: Biotin
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (96.14kD).)

Western Blot (WB)

(PCK2 monoclonal antibody. Western Blot analysis of PCK2 expression in A-431.)

Testing Data

(Detection limit for recombinant GST tagged PCK2 is 3ng/ml as a capture antibody.)

Related Product Information for anti-PEPCK2 antibody

This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described.

Product Categories/Family for anti-PEPCK2 antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 34783904
• NCBI GeneID: 5106
• Molecular Weight: 47,564 Da
• NCBI Official Full Name: Homo sapiens phosphoenolpyruvate carboxykinase 2 (mitochondrial), mRNA
• NCBI Official Synonym Full Names: phosphoenolpyruvate carboxykinase 2, mitochondrial
• NCBI Official Symbol: PCK2
• NCBI Official Synonym Symbols: PEPCK; PEPCK2; PEPCK-M
• NCBI Protein Information: phosphoenolpyruvate carboxykinase [GTP], mitochondrial
• Protein Family: PPi-type phosphoenolpyruvate carboxykinase

NCBI Description

This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2014]