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Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cell using MRPL28 antibody. Blue: DAPI for nuclear staining.)

Rabbit MRPL28 Polyclonal Antibody | anti-MRPL28 antibody

MRPL28 (Mitochondrial Ribosomal Protein L28, p15, MAAT1) (APC)

Gene Names
MRPL28; p15; MAAT1
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Western Blot, Immunohistochemistry
Purity
Purified by affinity chromatography.
Synonyms
MRPL28; Polyclonal Antibody; MRPL28 (Mitochondrial Ribosomal Protein L28; p15; MAAT1) (APC); anti-MRPL28 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes MRPL28. Species Crossreactivity: human, mouse, rat
Purity/Purification
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Concentration
~1mg/ml (varies by lot)
Applicable Applications for anti-MRPL28 antibody
Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
IF: 1:50-1:200
WB: 1:500-1:2000
IHC: 1:50-1:200
Applications are based on unconjugated antibody.
Immunogen
Recombinant protein corresponding to human MRPL28.
Conjugate
APC
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using MRPL28 antibody. Blue: DAPI for nuclear staining.)

Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cell using MRPL28 antibody. Blue: DAPI for nuclear staining.)

Testing Data

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Testing Data ()
Product Categories/Family for anti-MRPL28 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
30,157 Da
NCBI Official Full Name
39S ribosomal protein L28, mitochondrial
NCBI Official Synonym Full Names
mitochondrial ribosomal protein L28
NCBI Official Symbol
MRPL28
NCBI Official Synonym Symbols
p15; MAAT1
NCBI Protein Information
39S ribosomal protein L28, mitochondrial; L28mt; MRP-L28; melanoma antigen p15; melanoma-associated antigen recognised by cytotoxic T lymphocytes; melanoma-associated antigen recognized by T lymphocytes; melanoma-associated antigen recognized by T-lymphoc
UniProt Protein Name
39S ribosomal protein L28, mitochondrial
Protein Family
UniProt Gene Name
MRPL28
UniProt Synonym Gene Names
MAAT1; L28mt; MRP-L28
UniProt Entry Name
RM28_HUMAN

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion. [provided by RefSeq, Jul 2008]

Uniprot Description

MRPL28: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion. [provided by RefSeq, Jul 2008]

Protein type: Ribosomal; Translation; Mitochondrial

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; mitochondrial inner membrane; mitochondrial ribosome

Molecular Function: protein binding; structural constituent of ribosome

Biological Process: translation; mitochondrial translation; organelle organization and biogenesis

Research Articles on MRPL28

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Product Notes

The MRPL28 mrpl28 (Catalog #AAA6427256) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MRPL28 (Mitochondrial Ribosomal Protein L28, p15, MAAT1) (APC) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MRPL28 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC). IF: 1:50-1:200 WB: 1:500-1:2000 IHC: 1:50-1:200 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MRPL28 mrpl28 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MRPL28, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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