Rabbit anti-Human NASP Polyclonal Antibody | anti-NASP antibody

Anti-NASP Antibody, Rabbit Polyclonal

Cat. Num. AAA8108172

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: NASP; Polyclonal Antibody; Anti-NASP Antibody; Rabbit Polyclonal; NASP Antibody; Rabbit PAb; Antigen Affinity Purified; nuclear autoantigenic sperm protein (histone-binding); Anti-FLB7527 Antibody; Anti-PRO1999 Antibody; anti-NASP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human NASP
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-NASP antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:2000-1:10,000; IHC-P: 1:500-1:5000; ICC/IF: 1:100-1:500; IP: 1-5uL/mg of lysate
• Immunogen: E Coli-derived Human NASP fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human NASP fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-NASP rabbit polyclonal antibody at 1:2000 dilution Lane A: 293T Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: HeLa Whole Cell Lysate Lane D: HEK293 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:86 kDa Observed band size:180 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of human NASP in human rectum with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)

Immunofluorescence (IF)

(Immunofluorescence staining of NASP in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human NASP polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)

Immunoprecipitation (IP)

(NASP was immunoprecipitated using: Lane A:0.5 mg 293T Whole Cell Lysate 4 uL anti-NASP rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-NASP rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 86 kDa Observed band size :180 kDa)

Related Product Information for anti-NASP antibody

NASP (Nuclear Autoantigenic Sperm Protein) is a linker histone chaperone required for normal cell division. NASP is a histone H1 binding protein expressed in all cells undergoing division. Synchronized HeLa cells are delayed in progression through the G1/S border when transiently transfected to overexpress full-length NASP, but not the histone-binding site (HBS) deletion mutant (NASP-DeltaHBS). Changes in NASP expression significantly affect cell growth and development; loss of gene function results in embryonic lethality. In humans, growing evidence implicates NASP miss-regulation in the development of a variety of cancers. NASP cDNA contains 2561 nt encoding a protein of 787 amino acids. The open reading frame contains 2446 nt followed by an ochre stop codon (TAA) and 104 nucleotides of untranslated sequence containing a poly(A) addition signal 10 bases upstream of the poly(A) tail.

References

O'Rand MG, et al. (1992) Sequence and localization of human nasp: Conservation of a xenopus histone-binding protein. Dev Biol 154 (1): 37-44.Alekseev OM, et al. (2009) Analysis of gene expression profiles in hela cells in response to overexpression or sirna-mediated depletion of nasp. Reprod Biol Endocrinol 7 45.Alekseev OM, et al. (2005) Mass spectrometry identification of nasp binding partners in hela cells. Proteins 61 (1): 1-5.