Rabbit PEA-15 Polyclonal Antibody | anti-PEA-15 antibody

PEA-15 (D110) Polyclonal Antibody

Cat. Num. AAA3001322

• Gene Names: PEA15; PED; MAT1; HMAT1; MAT1H; PEA-15; HUMMAT1H; PED-PEA15; PED/PEA15
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PEA-15; Polyclonal Antibody; PEA-15 (D110) Polyclonal Antibody; Astrocytic phosphoprotein PEA-15; 15 kDa phosphoprotein enriched in astrocytes; Phosphoprotein enriched in diabetes; PED; PEA15; anti-PEA-15 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PEA-15 (D110) polyclonal antibody detects endogenous levels of PEA-15 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 130

• Applicable Applications for anti-PEA-15 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 78-132 of Human PEA-15.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of PEA-15 (D110) polyclonal antibody at 1:500 dilution Lane1:NIH-3T3 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:C6 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of PEA-15 (D110) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-PEA-15 antibody

PEA-15 (phosphoprotein enriched in astrocytes) exists in an non-phosphorylated form (N), and two phosphorylated forms, Pa and Pb. PEA-15 is an endogenous substrate for PKC, which mediates the transition from Pa to Pb. The level of PEA-15 phosphorylation changes upon depolymerization or stabilization of tubulins, indicating that PEA-15 co-localizes with microtubules. The first 80 amino acids of PEA-15 correspond to the death effector domain (DED), which is a domain found in proteins that regulate apoptotic signaling pathways. The DED domain is necessary for PEA-15 to block Ras suppression. Although PEA-15 is predominantly expressed in the central nervous system, low levels of PEA-15 are expressed in liver and kidney, and higher levels in muscle.PEA-15 is also referred to as PED, phosphoprotein enriched in diabetes, for its elevated expression in type 2 diabetic patients

NCBI and Uniprot Product Information

• NCBI GI #: 4505705
• NCBI GeneID: 8682
• NCBI Accession #: NP_003759.1
• NCBI GenBank Nucleotide #: NP_003759.1
• UniProt Accession #: Q15121
• Molecular Weight: ~ 36kDa
• NCBI Official Full Name: astrocytic phosphoprotein PEA-15 isoform b
• NCBI Official Synonym Full Names: proliferation and apoptosis adaptor protein 15
• NCBI Official Symbol: PEA15
• NCBI Official Synonym Symbols: PED; MAT1; HMAT1; MAT1H; PEA-15; HUMMAT1H; PED-PEA15; PED/PEA15
• NCBI Protein Information: astrocytic phosphoprotein PEA-15
• UniProt Protein Name: Astrocytic phosphoprotein PEA-15
• UniProt Gene Name: PEA15
• UniProt Synonym Gene Names: PED
• UniProt Entry Name: PEA15_HUMAN

NCBI Description

This gene encodes a death effector domain-containing protein that functions as a negative regulator of apoptosis. The encoded protein is an endogenous substrate for protein kinase C. This protein is also overexpressed in type 2 diabetes mellitus, where it may contribute to insulin resistance in glucose uptake. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

PEA-15: an ubiquitously expressed anti-apoptotic protein. Inhibits both Fas- and Tnfrsf1a/Tnfr1-mediated caspase-8 activity and apoptosis. Interacts with CASP8/FLICE and FADD. Regulates glucose transport by controlling both the content of GLUT1 glucose transporters on the plasma membrane and the insulin-dependent trafficking of GLUT4 from the cell interior to the surface. Most abundant in tissues such as heart, brain, muscle and adipose tissue which utilize glucose as an energy source. Lower expression in glucose-producing tissues. Higher levels of expression are found in tissues from individuals with type 2 diabetes than in controls.

Protein type: Motility/polarity/chemotaxis; Apoptosis

Chromosomal Location of Human Ortholog: 1q21.1

Cellular Component: microtubule associated complex; cytoplasm

Molecular Function: protein binding

Biological Process: negative regulation of glucose import; apoptosis; transport; DNA damage checkpoint; carbohydrate transport; response to morphine