Mouse PEA-15 Monoclonal Antibody | anti-PEA-15 antibody

PEA-15

Cat. Num. AAA556094

• Gene Names: PEA15; PED; MAT1; HMAT1; MAT1H; PEA-15; HUMMAT1H
• Reactivity: Human, Mouse, Rat, Bovine
• Applications: Immunocytochemistry, Western Blot, Immunofluorescence
• Synonyms: PEA-15; Monoclonal Antibody; anti-PEA-15 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Bovine
• Clonality: Monoclonal
• Isotype: Mouse IgG
• Form/Format: Affinity Purified antibody; Liquid. Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3

• Applicable Applications for anti-PEA-15 antibody: Immunocytochemistry (ICC), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: IF: 1:1,000-2,000; ICC: 1:1,000-2,000; WB: 1:1,000-2,000
• Immunogen: Full length human PEA-15 as expressed in and purified from E Coli
• Preparation and Storage: Antibody can also be aliquotted and stored frozen at -20 degree C in a manual defrost freezer for six months without detectable loss of activity. The antibody is stableat 2-8 degree C for 1 year. Avoid repeated freeze-thaw cycles.

Immunofluorescence (IF)

(Immunofluorescent analysis of rat brain section stained with mouse mAb to PEA-15, MO22196, dilution 1:1,000 in red, and costained with chicken pAb to MAP2, dilution 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. The PEA-15 antibody labels the cytoplasm of certain presumably neuronal cells which are not labelled by the astrocyte specific GFAP antibody.)

Western Blot (WB)

(Western blot analysis of different tissue lysates using mouse mAb to PEA-15 dilution 1:1,000 in green: [1] protein standard (red), [2] rat whole brain, [3] rat cerebellum, [4] mouse whole brain, [5] cow cortex and [6] cow cerebellum. The strong band at about 15kDa corresponds to the PEA-15 protein.)

Related Product Information for anti-PEA-15 antibody

PEA-15 was originally isolated as a major low molecular weight of embryonic mouse striatal astrocytes grown in cell culture. Three spots on 2D gels with an apparent molecular weight of 15kDa and isoelectric point 5.1-5.3 were shown to be different forms of one protein. The protein was serine phosphorylated on one site by protein kinase C both in vivo and in vitro and the protein was named "phosphoprotein enriched in astrocytes of 15kDa", hence PEA-15. Subsequent cloning and sequencing revealed a protein well conserved in sequence between mouse and human and which was heavily expressed in brain. Independently the same protein was found to be upregulated in fibroblasts and tissues of diabetic patients, and has hence named "protein enriched in diabetes" or PED. Immunocytochemical studies showed that the protein was heavily expressed in astrocytes and certain neurons in the CNS of mice, though it is expressed a lower levels ubiquitously. PEA-15 was shown to interact with extracellular signal regulated kinase and regulate the nuclear entry of this protein, and several other important interactions with other proteins involved in regulation of apoptosis, glucose metabolism and cell growth have been described. MCA-4D2 was made against a recombinant full length PEA-15 construct expressed in and purified from E Coli.

NCBI and Uniprot Product Information

• NCBI GI #: 4505705
• NCBI GeneID: 8682
• NCBI Accession #: NP_003759.1
• NCBI GenBank Nucleotide #: NM_003768.3
• UniProt Accession #: Q15121; O00511
• Molecular Weight: 15,040 Da
• NCBI Official Full Name: astrocytic phosphoprotein PEA-15
• NCBI Official Synonym Full Names: phosphoprotein enriched in astrocytes 15
• NCBI Official Symbol: PEA15
• NCBI Official Synonym Symbols: PED; MAT1; HMAT1; MAT1H; PEA-15; HUMMAT1H
• NCBI Protein Information: astrocytic phosphoprotein PEA-15; homolog of mouse MAT-1 oncogene; phosphoprotein enriched in diabetes; Phosphoprotein enriched in astrocytes, 15kD; 15 kDa phosphoprotein enriched in astrocytes
• UniProt Protein Name: Astrocytic phosphoprotein PEA-15
• UniProt Gene Name: PEA15
• UniProt Synonym Gene Names: PED
• UniProt Entry Name: PEA15_HUMAN

NCBI Description

This gene encodes a death effector domain-containing protein, which is a major phosphoprotein in astrocytes, and an endogenous substrate for protein kinase C. Studies using knockout mice suggest that this protein may protect astrocytes from TNF-induced apoptosis. This protein is also overexpressed in type 2 diabetes mellitus, where it may contribute to insulin resistance in glucose uptake. [provided by RefSeq, Sep 2011]

Uniprot Description

PEA-15: an ubiquitously expressed anti-apoptotic protein. Inhibits both Fas- and Tnfrsf1a/Tnfr1-mediated caspase-8 activity and apoptosis. Interacts with CASP8/FLICE and FADD. Regulates glucose transport by controlling both the content of GLUT1 glucose transporters on the plasma membrane and the insulin-dependent trafficking of GLUT4 from the cell interior to the surface. Most abundant in tissues such as heart, brain, muscle and adipose tissue which utilize glucose as an energy source. Lower expression in glucose-producing tissues. Higher levels of expression are found in tissues from individuals with type 2 diabetes than in controls.

Protein type: Motility/polarity/chemotaxis; Apoptosis

Chromosomal Location of Human Ortholog: 1q21.1

Cellular Component: microtubule associated complex; cytoplasm

Molecular Function: protein binding

Biological Process: negative regulation of glucose import; apoptosis; transport; DNA damage checkpoint; carbohydrate transport; response to morphine