CD46 recombinant protein

CD46 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121129

• Gene Names: CD46; MCP; TLX; AHUS2; MIC10; TRA2.10
• Purity: >95% as determined by SDS-PAGE
• Synonyms: CD46; CD46 Protein; Human; Recombinant (His Tag); Human CD46 Protein (His Tag); AHUS2 Protein; MCP Protein; MIC10 Protein; TLX Protein; TRA2.10 Protein; CD46 molecule; complement regulatory protein; CD46 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asp328

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Cys 35
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human CD46 (EAW93471.1) extracellular domain (Met 1-Asp 328) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CD46 recombinant protein

Background: CD46, also known as Membrane Cofactor Protein (MCP), is a complement regulatory protein. CD46 is a type 1 membrane protein that plays an important inhibitory role in the complement system. CD46 is expressed in white blood cells, platelets, epithelial cells, and fibroblasts. Human CD46 shares 5% amino acid sequence identity with mouse and rat CD46. The importance of CD46 to complement regulation is underscored by the observation that genetic loss of CD46 leads to development of atypical hemolytic-uremic syndrome (aHUS), a disease characterized by uncontrolled complement activation. CD46 is implicated in the development and/or progression of selected cancer types.

Product Categories/Family for CD46 recombinant protein

Complement Activation Pathways

References

Lublin D.M., et al.,(1988), Molecular cloning and chromosomal localization of human membrane cofactor protein (MCP). Evidence for inclusion in the multigene family of complement-regulatory proteins. J. Exp. Med. 168:181-194.Purcell D.F., et al., (1991), Alternatively spliced RNAs encode several isoforms of CD46 (MCP), a regulator of complement activation.Immunogenetics 33:335-344.Post T.W., et al.,(1991), Membrane cofactor protein of the complement system: alternative splicing of serine/threonine/proline-rich exons and cytoplasmic tails produces multiple isoforms that correlate with protein phenotype.J. Exp. Med. 174:93-102.

NCBI and Uniprot Product Information

• NCBI GI #: 119613877
• NCBI GeneID: 4179
• Molecular Weight: 36,826 Da
• NCBI Official Full Name: CD46 antigen, complement regulatory protein, isoform CRA_i
• NCBI Official Synonym Full Names: CD46 molecule
• NCBI Official Symbol: CD46
• NCBI Official Synonym Symbols: MCP; TLX; AHUS2; MIC10; TRA2.10
• NCBI Protein Information: membrane cofactor protein
• Protein Family: Membrane cofactor protein

NCBI Description

The protein encoded by this gene is a type I membrane protein and is a regulatory part of the complement system. The encoded protein has cofactor activity for inactivation of complement components C3b and C4b by serum factor I, which protects the host cell from damage by complement. In addition, the encoded protein can act as a receptor for the Edmonston strain of measles virus, human herpesvirus-6, and type IV pili of pathogenic Neisseria. Finally, the protein encoded by this gene may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations at this locus have been associated with susceptibility to hemolytic uremic syndrome. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2010]