Rabbit anti-Human PDPK1 Ser396 Polyclonal Antibody | anti-PDPK1 antibody

PDPK1, phosphorylated Ser396 (3-phosphoinositide Dependent Protein Kinase-1, hPDK1, PDK1)

Cat. Num. AAA623897

• Gene Names: PDPK1; PDK1; PRO0461; MGC20087; MGC35290
• Reactivity: Human
• Applications: ELISA, Gel Super Shift Assay
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: PDPK1 Ser396; Polyclonal Antibody; PDPK1; phosphorylated Ser396 (3-phosphoinositide Dependent Protein Kinase-1; hPDK1; PDK1); Anti -PDPK1; anti-PDPK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PDPK1 when phosphorylated at Ser393.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-PDPK1 antibody: ELISA (EL/EIA), Gel Shift Assay (GS/EMSA)
• Application Notes: Suitable for use in ELISA and Dot Blot.; Dilution: ELISA: 1:1,000; Dot Blot: 1:500
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser396 of human PDPK1 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of anti-PDPK1-pS396 Phospho-specific Pab (RB13093) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Related Product Information for anti-PDPK1 antibody

PDPK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDPK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDPK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis. Because of the role that PDPK1 plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.

Product Categories/Family for anti-PDPK1 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 49168520
• NCBI GeneID: 5170
• UniProt Accession #: O15530; Q6FI20; Q53HJ9; Q59EH6; Q9BRD5; Q9UPJ7; Q9UPJ8
• Molecular Weight: 63,152 Da
• NCBI Official Full Name: PDPK1
• NCBI Official Synonym Full Names: 3-phosphoinositide dependent protein kinase-1
• NCBI Official Symbol: PDPK1
• NCBI Official Synonym Symbols: PDK1; PRO0461; MGC20087; MGC35290
• NCBI Protein Information: 3-phosphoinositide-dependent protein kinase 1; hPDK1; PkB-like 1; OTTHUMP00000159109; OTTHUMP00000159110; OTTHUMP00000174525; PkB kinase like gene 1
• UniProt Protein Name: 3-phosphoinositide-dependent protein kinase 1
• Protein Family: 3-phosphoinositide-dependent protein kinase
• UniProt Gene Name: PDPK1
• UniProt Synonym Gene Names: PDK1
• UniProt Entry Name: PDPK1_HUMAN

Uniprot Description

PDK1: an AGC kinase of the PKB family that contains a PH domain. Involved in a wide variety of processes including cell proliferation, differentiation and apoptosis. Autophosphorylation in the activation loop is necessary for activity. Phosphorylates and activates kinases including Akt, PKC isozymes, p70 S6K and RSK. Three alternatively-spliced isoforms have been described.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Nuclear receptor co-regulator; EC 2.7.11.1; Protein kinase, AGC; AGC group; PKB family

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nucleoplasm; cell projection; focal adhesion; membrane; cytoplasmic membrane-bound vesicle; cytoplasm; plasma membrane; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; phospholipase binding; 3-phosphoinositide-dependent protein kinase activity; kinase activity; protein kinase binding; ATP binding; insulin receptor binding; phospholipase activator activity

Biological Process: regulation of mast cell degranulation; focal adhesion formation; nerve growth factor receptor signaling pathway; protein amino acid autophosphorylation; T cell receptor signaling pathway; protein amino acid phosphorylation; synaptic transmission; regulation of transcription, DNA-dependent; regulation of I-kappaB kinase/NF-kappaB cascade; epidermal growth factor receptor signaling pathway; platelet activation; cell migration; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; transcription, DNA-dependent; activation of protein kinase B; peptidyl-threonine phosphorylation; calcium-mediated signaling; hyperosmotic response; cellular response to insulin stimulus; T cell costimulation; negative regulation of toll-like receptor signaling pathway; insulin receptor signaling pathway; negative regulation of protein kinase activity; innate immune response; positive regulation of release of sequestered calcium ion into cytosol; actin cytoskeleton organization and biogenesis; blood coagulation; negative regulation of transforming growth factor beta receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway