Rabbit anti-Human PDLIM7 Polyclonal Antibody | anti-PDLIM7 antibody

PDLIM7 (PDZ and LIM Domain Protein 7, LIM Mineralization Protein, LMP, Protein Enigma, ENIGMA) (HRP)

Cat. Num. AAA6388936

• Gene Names: PDLIM7; LMP1; LMP3
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PDLIM7; Polyclonal Antibody; PDLIM7 (PDZ and LIM Domain Protein 7; LIM Mineralization Protein; LMP; Protein Enigma; ENIGMA) (HRP); anti-PDLIM7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PDLIM7.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PDLIM7 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human PDLIM7, aa1-457 (NP_005442.2)
• Immunogen Sequence: MDSFKVVLEGPAPWGFRLQGGKDFNVPLSISRLTPGGKAAQAGVAVGDWVLSIDGENAGSLTHIEAQNKIRACGERLSLGLSRAQPVQSKPQKASAPAADPPRYTFAPSVSLNKTARPFGAPPPADSAPQQNGQPLRPLVPDASKQRLMENTEDWRPRPGTGQSRSFRILAHLTGTEFMQDPDEEHLKKSSQVPRTEAPAPASSTPQEPWPGPTAPSPTSRPPWAVDPAFAERYAPDKTSTVLTRHSQPATPTPLQSRTSIVQAAAGGVPGGGSNNGKTPVCHQCHKVIRGRYLVALGHAYHPEEFVCSQCGKVLEEGGFFEEKGAIFCPPCYDVRYAPSCAKCKKKITGEIMHALKMTWHVHCFTCAACKTPIRNRAFYMEEGVPYCERDYEKMFGTKCHGCDFKIDAGDRFLEALGFSWHDTCFVCAICQINLEGKTFYSKKDRPLCKSHAFSHV
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of PDLIM7 expression in transfected 293T cell line by PDLIM7 polyclonal antibody. Lane 1: PDLIM7 transfected lysate (49.8kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-PDLIM7 antibody

PDLIM7 (PDZ and LIM domain 7) is a representative of a family of proteins composed of conserved PDZ and LIM domains. The LIM domains of this protein bind to protein kinases, whereas the PDZ domain binds to actin filaments. It is likely to act as a scaffold for the formation of multiprotein complexes. It may play a role as an adapter that, via its PDZ domain, localizes LIM-binding proteins to actin filaments of both skeletal muscle and nonmuscle tissues. PDLIM7 is involved in the fundamental mechanisms of bone formation and is involved in the BMP signaling pathway. There are six isoforms.

Product Categories/Family for anti-PDLIM7 antibody

Antibodies; PDZ Domain

NCBI and Uniprot Product Information

• NCBI GI #: 11496885
• NCBI GeneID: 9260
• NCBI Accession #: NP_005442.2
• NCBI GenBank Nucleotide #: NM_005451.3
• UniProt Accession #: Q9NR12; Q14250; Q5XG82; Q6NVZ5; Q96C91; Q9BXB8; Q9BXB9
• Molecular Weight: 24,413 Da
• NCBI Official Full Name: PDZ and LIM domain protein 7 isoform 1
• NCBI Official Synonym Full Names: PDZ and LIM domain 7 (enigma)
• NCBI Official Symbol: PDLIM7
• NCBI Official Synonym Symbols: LMP1; LMP3
• NCBI Protein Information: PDZ and LIM domain protein 7; LMP; 1110003B01Rik; protein enigma; Lim mineralization protein 3
• UniProt Protein Name: PDZ and LIM domain protein 7
• Protein Family: PDZ and LIM domain protein
• UniProt Gene Name: PDLIM7
• UniProt Synonym Gene Names: ENIGMA; LMP
• UniProt Entry Name: PDLI7_HUMAN

Uniprot Description

PDLIM7: May function as a scaffold on which the coordinated assembly of proteins can occur. May play a role as an adapter that, via its PDZ domain, localizes LIM-binding proteins to actin filaments of both skeletal muscle and nonmuscle tissues. Involved in both of the two fundamental mechanisms of bone formation, direct bone formation e.g (embryonic flat bones mandible and cranium), and endochondral bone formation e.g (embryonic long bone development). Plays a role during fracture repair. Involved in BMP6 signaling pathway. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 5q35.3

Cellular Component: ruffle; focal adhesion; cytoplasm; stress fiber; nucleus; actin cytoskeleton

Molecular Function: protein binding; zinc ion binding

Biological Process: receptor-mediated endocytosis; positive regulation of osteoblast differentiation; ossification; multicellular organismal development; cell differentiation; actin cytoskeleton organization and biogenesis