CGB7 recombinant protein

CGB7 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121277

• Gene Names: CGB; CGB3; CGB5; CGB7; CGB8; hCGB
• Purity: >96% as determined by SDS-PAGE
• Synonyms: CGB7; CGB7 Protein; Human; Recombinant (His Tag); Human CGB7 Protein (His Tag); CG-beta-a Protein; CGB6 Protein; chorionic gonadotropin beta subunit 7; CGB7 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >96% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH7.4, 10% gly. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gln165

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ser 21
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human CGB7 isoform 1 (P01233-1)(Met 1-Gln 165) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CGB7 recombinant protein

Background: CGB7 (chorionic gonadotropin, beta polypeptide 7) belongs to the glycoprotein hormones subunit beta family. Glycoprotein hormones are heterodimers consisting of a common alpha subunit and an unique beta subunit which confers biological specificity. CGB7 gene is a member of the glycoprotein hormone beta chain family and encodes the beta 7 subunit of chorionic gonadotropin (CG). CG is produced by the trophoblastic cells of the placenta and stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy. The beta subunit of CG is encoded by 6 genes which are arranged in tandem and inverted pairs on chromosome 19q13.3 and contiguous with the luteinizing hormone beta subunit gene. CGB7 is used as adjunctive therapy in the treatment of obesity. CGB7 also stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy.

References

Fiddes J.C., et al.,(1980), The cDNA for the beta-subunit of human chorionic gonadotropin suggests evolution of a gene by readthrough into the 3'-untranslated region. Nature 286:684-687.Talmadge K., et al., (1984), Evolution of the genes for the beta subunits of human chorionic gonadotropin and luteinizing hormone.Nature 307:37-40.Policastro P., et al.,(1983), The beta subunit of human chorionic gonadotropin is encoded by multiple genes.J. Biol. Chem. 258:11492-11499.

NCBI and Uniprot Product Information

• NCBI GI #: 4502789
• NCBI GeneID: 1082
• NCBI Accession #: NP_000728.1
• NCBI GenBank Nucleotide #: NM_000737.3
• UniProt Accession #: P01233; Q13991; Q14000; Q3KPI3; Q3SY41; Q8WTT5; Q8WXL1; Q8WXL2; Q8WXL3; Q8WXL4; A1A5E0; B9ZVP5
• Molecular Weight: 17,739 Da
• NCBI Official Full Name: choriogonadotropin subunit beta
• NCBI Official Synonym Full Names: chorionic gonadotropin, beta polypeptide
• NCBI Official Symbol: CGB
• NCBI Official Synonym Symbols: CGB3; CGB5; CGB7; CGB8; hCGB
• NCBI Protein Information: choriogonadotropin subunit beta; CG-beta; chorionic gonadotropin beta chain; chorionic gonadotrophin chain beta; chorionic gonadotropin beta subunit; chorionic gonadotropin beta 3 subunit
• UniProt Protein Name: Choriogonadotropin subunit beta
• Protein Family: Choriogonadotropin
• UniProt Gene Name: CGB
• UniProt Synonym Gene Names: CGB3; CG-beta
• UniProt Entry Name: CGHB_HUMAN

NCBI Description

This gene is a member of the glycoprotein hormone beta chain family and encodes the beta 3 subunit of chorionic gonadotropin (CG). Glycoprotein hormones are heterodimers consisting of a common alpha subunit and an unique beta subunit which confers biological specificity. CG is produced by the trophoblastic cells of the placenta and stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy. The beta subunit of CG is encoded by 6 genes which are arranged in tandem and inverted pairs on chromosome 19q13.3 and contiguous with the luteinizing hormone beta subunit gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CGB: Stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy. Belongs to the glycoprotein hormones subunit beta family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Inhibitor; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: extracellular region

Molecular Function: hormone activity

Biological Process: cellular protein metabolic process; cell-cell signaling; apoptosis; peptide hormone processing; female gamete generation; signal transduction