Rabbit anti-Mouse, Human PDGF-D Polyclonal Antibody | anti-PDGFD antibody

Anti-PDGF-D Antibody, Rabbit Polyclonal

Cat. Num. AAA8103469

• Gene Names: Pdgfd; 1110003I09Rik
• Reactivity: Mouse, Human
• Applications: Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: PDGF-D; Polyclonal Antibody; Anti-PDGF-D Antibody; Rabbit Polyclonal; PDGF-D/PDGFD Antibody; Rabbit PAb; Antigen Affinity Purified; platelet derived growth factor D; Anti-1110003I09Rik Antibody; anti-PDGFD antibody

• Host: Rabbit
• Reactivity: Mouse, Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse PDGF-D
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-PDGFD antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:200-1:1000
• Immunogen: A synthetic peptide corresponding to the center region of the Mouse PDGF-D/PDGFD
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Mouse PDGF-D/PDGFD, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining PDGFD in mouse kidney with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining PDGFD in mouse heart with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining PDGFD in human kidney with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-PDGFD antibody

Platelet-derived growth factor D (PDGF-D), also known as Iris-expressed growth factor, is a member of the PDGF/vascular endothelial growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines, seven of which are found in this factor. PDGF-D/PDGFD only forms homodimers and, therefore, does not dimerize with the other three family members. It differs from alpha and beta members of this family in having an unusual N-terminal domain, the CUB domain. The expression of PDGF-D/PDGFD in the eye is tissue-specific. In the anterior segment, it is localized to iris and ciliary body, whereas in the retina, PDGF-D/PDGFD is restricted to the outer plexiform layer. PDGF-D/PDGFD is present in aqueous humor but is not detectable in mature lens or in mouse lens-derived alphaTN4-1 cells. PDGF-D/PDGFD is highly expressed in human breast cancer and facilitates tumor growth and lymph node metastasis, making it a potential target in breast cancer. PDGF-D/PDGFD increases drug delivery and hence improves the efficacy of chemotherapy through vessel normalization. Intervention in the PDGF-D pathway in the eye, perhaps by antibody or blocking peptide, could be useful in the treatment of certain cataracts, including post-operative secondary cataract.

References

Ray S, et al. (2005) Platelet-derived growth factor D, tissue-specific expression in the eye, and a key role in control of lens epithelial cell proliferation. J Biol Chem. 280(9): 8494-502.Uutela M, et al. (2004) PDGF-D induces macrophage recruitment, increased interstitial pressure, and blood vessel maturation during angiogenesis. Blood. 104 (10): 3198-204.Taneda S, et al. (2004) Obstructive uropathy in mice and humans: potential role for PDGF-D in the progression of tubulointerstitial injury. J Am Soc Nephrol. 14 (10): 2544-55.

NCBI and Uniprot Product Information

• NCBI GI #: 27229137
• NCBI GeneID: 71785
• NCBI Accession #: NP_082200.1
• NCBI GenBank Nucleotide #: NM_027924.2
• UniProt Accession #: Q925I7; Q3URF6; Q8K2L3; Q9D1L8
• Molecular Weight: 42,809 Da
• NCBI Official Full Name: platelet-derived growth factor D
• NCBI Official Synonym Full Names: platelet-derived growth factor, D polypeptide
• NCBI Official Symbol: Pdgfd
• NCBI Official Synonym Symbols: 1110003I09Rik
• NCBI Protein Information: platelet-derived growth factor D; PDGF-D; SCDGF-B; spinal cord-derived growth factor B
• UniProt Protein Name: Platelet-derived growth factor D
• Protein Family: Platelet-derived growth factor
• UniProt Gene Name: Pdgfd
• UniProt Synonym Gene Names: Scdgfb; PDGF-D; SCDGF-B; PDGFD latent form; PDGFD receptor-binding form
• UniProt Entry Name: PDGFD_MOUSE

Uniprot Description

PDGFD: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Plays an important role in wound healing. Induces macrophage recruitment, increased interstitial pressure, and blood vessel maturation during angiogenesis. Can initiate events that lead to a mesangial proliferative glomerulonephritis, including influx of monocytes and macrophages and production of extracellular matrix. Belongs to the PDGF/VEGF growth factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Oncoprotein

Cellular Component: extracellular space; membrane; extracellular region

Molecular Function: growth factor activity; platelet-derived growth factor receptor binding

Biological Process: multicellular organismal development; positive regulation of cell division; platelet-derived growth factor receptor signaling pathway; cellular process; regulation of peptidyl-tyrosine phosphorylation