Mouse platelet derived growth factor DD ELISA Kit | PDGF-DD elisa kit

Mouse Platelet-derived growth factor DD, PDGF-DD ELISA Kit

Cat. Num. AAA924348

• Gene Names: Pdgfd; 1110003I09Rik
• Reactivity: Mouse
• Synonyms: platelet derived growth factor DD; Mouse Platelet-derived growth factor DD; PDGF-DD ELISA Kit; Mouse Platelet-derived growth factor DD (PDGF-DD) ELISA kit; PDGF-DD elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse PDGF-DD. No significant cross-reactivity or interference between mouse PDGF-DD and analogues was observed.
• Sequence Length: 370

• Samples: Serum, plasma, tissue homogenates.
• Assay Type: Sandwich
• Detection Range: 0.156 ng/ml -10 ng/ml.
• Sensitivity: 0.039 ng/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PDGF-DD elisa kit

Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PDGF-DD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PDGF-DD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDGF-DD is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDGF-DD bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 27229137
• NCBI GeneID: 71785
• NCBI Accession #: NP_082200.1
• NCBI GenBank Nucleotide #: NM_027924.2
• UniProt Accession #: Q925I7; Q3URF6; Q8K2L3; Q9D1L8
• Molecular Weight: 42,809 Da
• NCBI Official Full Name: platelet-derived growth factor D
• NCBI Official Synonym Full Names: platelet-derived growth factor, D polypeptide
• NCBI Official Symbol: Pdgfd
• NCBI Official Synonym Symbols: 1110003I09Rik
• NCBI Protein Information: platelet-derived growth factor D; PDGF-D; SCDGF-B; spinal cord-derived growth factor B
• UniProt Protein Name: Platelet-derived growth factor D
• UniProt Gene Name: Pdgfd
• UniProt Synonym Gene Names: Scdgfb; PDGF-D; SCDGF-B; PDGFD latent form; PDGFD receptor-binding form
• UniProt Entry Name: PDGFD_MOUSE

Uniprot Description

PDGFD: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Plays an important role in wound healing. Induces macrophage recruitment, increased interstitial pressure, and blood vessel maturation during angiogenesis. Can initiate events that lead to a mesangial proliferative glomerulonephritis, including influx of monocytes and macrophages and production of extracellular matrix. Belongs to the PDGF/VEGF growth factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Oncoprotein

Cellular Component: extracellular space; membrane; extracellular region

Molecular Function: growth factor activity; platelet-derived growth factor receptor binding

Biological Process: multicellular organismal development; positive regulation of cell division; platelet-derived growth factor receptor signaling pathway; cellular process; regulation of peptidyl-tyrosine phosphorylation