Human Growth Differentiation Factor 7 ELISA Kit | GDF7 elisa kit

Human Growth Differentiation Factor 7 ELISA Kit

Cat. Num. AAA765299

• Gene Names: GDF7; BMP12
• Reactivity: Human
• Synonyms: Growth Differentiation Factor 7; Human Growth Differentiation Factor 7 ELISA Kit; GDF7/BMP-12/BMP12/GDF-7/growth differentiation factor 7/growth/differentiation factor 7; GDF7 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of GDF3. No significant cross-reactivity or interference between GDF3 and analogues was observed.
• Sequence Length: 453

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 78.125-5000pg/ml
• Sensitivity: <46.875pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GDF3 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GDF3 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GDF7 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-GDF3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-GDF3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the GDF3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of GDF3 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 40786371
• NCBI GeneID: 151449
• Molecular Weight: 46,950 Da
• NCBI Official Full Name: growth differentiation factor 7
• NCBI Official Synonym Full Names: growth differentiation factor 7
• NCBI Official Symbol: GDF7
• NCBI Official Synonym Symbols: BMP12
• NCBI Protein Information: growth/differentiation factor 7
• UniProt Protein Name: Growth/differentiation factor 7
• Protein Family: Growth/differentiation factor
• UniProt Gene Name: GDF7
• UniProt Synonym Gene Names: GDF-7
• UniProt Entry Name: GDF7_HUMAN

NCBI Description

This gene encodes a member of the bone morphogenetic protein (BMP) family. BMPs belong to the transforming growth factor-beta superfamily of secreted signalling molecules that regulate diverse processes in growth, repair and embryonic development. In mouse, this gene functions as an inductive signal from the roof plate required for the specification of neuronal identity in the dorsal spinal cord. [provided by RefSeq, Jul 2008]

Uniprot Description

GDF7: May play an active role in the motor area of the primate neocortex. Belongs to the TGF-beta family.

Protein type: Secreted; Cytokine; Cell development/differentiation; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2p24.1

Cellular Component: extracellular space

Molecular Function: cytokine activity; growth factor activity; protein binding; protein homodimerization activity; transforming growth factor beta receptor binding

Biological Process: activin receptor signaling pathway; axon guidance; BMP signaling pathway; branching morphogenesis of a tube; cell development; cell fate commitment; epithelial cell differentiation; forebrain morphogenesis; gland morphogenesis; growth; midbrain development; positive regulation of neuron differentiation; positive regulation of transcription, DNA-dependent; regulation of apoptosis; regulation of MAPKKK cascade; reproductive structure development; roof plate formation; spinal cord association neuron differentiation