Rabbit anti-Mouse PDE8B Polyclonal Antibody | anti-PDE8B antibody

PDE8B, ID (PDE8B, High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B, Cell proliferation-inducing gene 22 protein) (FITC)

Cat. Num. AAA6332235

• Gene Names: Pde8b; B230331L10Rik; C030047E14Rik
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PDE8B; Polyclonal Antibody; ID (PDE8B; High affinity cAMP-specific and IBMX-insensitive 3'; 5'-cyclic phosphodiesterase 8B; Cell proliferation-inducing gene 22 protein) (FITC); Cell proliferation-inducing gene 22 protein; anti-PDE8B antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-PDE8B antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PDE8B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 296-324 amino acids from the Central region of human PDE8B.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PDE8B Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the PDE8B antibody detected the PDE8B protein (arrow).)

Related Product Information for anti-PDE8B antibody

The protein encoded by this gene is a cyclic nucleotide phosphodiesterase (PDE) that catalyzes the hydrolysis of the second messenger cAMP. The encoded protein, which does not hydrolyze cGMP, is resistant to several PDE inhibitors. Defects in this gene are a cause of autosomal dominant striatal degeneration (ADSD). Several transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-PDE8B antibody

Antibodies; Enzymes; Phosphodiesterase

References

Horvath, A., et al. Thyroid 20(4):363-367(2010). Rose, J. Phd, et al. Mol. Med. (2010). In press: Appenzeller, S., et al. Am. J. Hum. Genet. 86(1):83-87(2010). Shields, B.M., et al. J. Clin. Endocrinol. Metab. 94(11):4608-4612(2009). Bimpaki, E.I., et al. Eur. J. Endocrinol. 161(1):153-161(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 363805461
• NCBI GeneID: 218461
• UniProt Accession #: O95263; E9Q4S1
• Molecular Weight: 96,739 Da
• NCBI Official Full Name: High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B
• NCBI Official Synonym Full Names: phosphodiesterase 8B
• NCBI Official Symbol: Pde8b
• NCBI Official Synonym Symbols: B230331L10Rik; C030047E14Rik
• NCBI Protein Information: high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B
• UniProt Protein Name: High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B
• Protein Family: High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase
• UniProt Gene Name: PDE8B
• UniProt Synonym Gene Names: HsPDE8B
• UniProt Entry Name: PDE8B_HUMAN

Uniprot Description

PDE8B: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in specific signaling in the thyroid gland. Defects in PDE8B are the cause of striatal degeneration autosomal dominant (ADSD). It is a movement disorder affecting the striatal part of the basal ganglia and characterized by bradykinesia, dysarthria and muscle rigidity. These symptoms resemble idiopathic Parkinson disease, but tremor is not present. Defects in PDE8B are the cause of primary pigmented nodular adrenocortical disease type 3 (PPNAD3). PPNAD3 is a rare bilateral adrenal defect causing ACTH-independent Cushing syndrome. Macroscopic appearance of the adrenals is characteristic with small pigmented micronodules observed in the cortex. Adrenal glands show overall normal size and weight, and multiple small yellow-to-dark brown nodules surrounded by a cortex with a uniform appearance. Microscopically, there are moderate diffuse cortical hyperplasia with mostly nonpigmented nodules, multiple capsular deficits and massive circumscribed and infiltrating extra-adrenal cortical excrescences with micronodules. Clinical manifestations of Cushing syndrome include facial and trunkal obesity, abdominal striae, muscular weakness, osteoporosis, arterial hypertension, diabetes. Belongs to the cyclic nucleotide phosphodiesterase family. PDE8 subfamily. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleotide Metabolism - purine; Phosphodiesterase; EC 3.1.4.53

Chromosomal Location of Human Ortholog: 5q13.3

Cellular Component: cytosol

Molecular Function: 3',5'-cyclic-AMP phosphodiesterase activity; signal transducer activity; metal ion binding; 3',5'-cyclic-nucleotide phosphodiesterase activity

Biological Process: cyclic nucleotide metabolic process; behavioral fear response; operant conditioning; cAMP catabolic process; two-component signal transduction system (phosphorelay); visual learning; neuromuscular process controlling balance; negative regulation of insulin secretion

Disease: Striatal Degeneration, Autosomal Dominant; Pigmented Nodular Adrenocortical Disease, Primary, 3