5'-Nucleotidase, Ecto (NT5E) Recombinant Protein | NT5E recombinant protein

Recombinant 5'-Nucleotidase, Ecto (NT5E)

Cat. Num. AAA2010357

• Gene Names: Nt5e; Nt5; CD73
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: 5'-Nucleotidase; Ecto (NT5E); Recombinant 5'-Nucleotidase; NT5E recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-WE LTIMHTNDVH SRLEQTSDDS TKCLNASLCV GGVARLFTKV QQIRKEEPNV LLLDAGDQYQ GTIWFTVYKG LEVAHFMNLL GYDAMALGNH EFDNGVEGLI DPLLRNVKFP ILSANIKARG PLAPQISGLY LPYKVLSVGG EVVGIVGYTS KETPFLSNPG TNLVFEDEVT ALQPEVDKLK TLNVNKIIAL GHSGFEMDKL IAQKVRGVDV VVGGHTNTFL YTGNPPSKEV PAGKYPFIVT SDDGRKVPVV QAYAFGKYLG YLKVEFDDKG NVVTSYGNPI LLNSTIREDA AIKADINQWR IKLDNYSTQE LGRTIVYLNG SAQECRFREC NMGNLICDAM INNNLRHPDE MFWNHVSMCI VNGGGIRSPI DERNNGTITW ENLAAVLPFG GTFDLVQLKG STLKKAFEHS VHRYGQSTGE FLQVGGIHVV YDISRKPWDR VVQLKVLCTK CRVPIYEPLE MDKVYKVVLP SYLVNGGDGF QMIKDELLKH DSGDQDISVV SEYISKMKVI YPAVEGRIKF
• Sequence Length: 576

• Applicable Applications for NT5E recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Trp29~phe550 (Accession # P21588) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 112826
• NCBI GeneID: 58813
• UniProt Accession #: P21588
• Molecular Weight: 59.6kDa
• NCBI Official Full Name: 5'-nucleotidase
• NCBI Official Synonym Full Names: 5' nucleotidase, ecto
• NCBI Official Symbol: Nt5e
• NCBI Official Synonym Symbols: Nt5; CD73
• NCBI Protein Information: 5'-nucleotidase; 5'-NT; ecto-5'-nucleotidase
• UniProt Protein Name: 5'-nucleotidase
• UniProt Gene Name: Nt5e
• UniProt Synonym Gene Names: Nt5; Nte; 5'-NT
• UniProt Entry Name: 5NTD_RAT

NCBI Description

may hydrolyze extracellular nucleotides into membrane permeable nucleosides [RGD, Feb 2006]

Uniprot Description

NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5'-nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.3.5; Nucleotide Metabolism - pyrimidine; Membrane protein, integral; Nucleotide Metabolism - purine; Phosphatase (non-protein); Membrane protein, GPI anchor; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide

Cellular Component: cell surface; membrane; cytoplasm; plasma membrane; integral to membrane

Molecular Function: 5'-nucleotidase activity; metal ion binding; ferrous iron binding; nucleotide binding

Biological Process: dephosphorylation; negative regulation of inflammatory response; adenosine biosynthetic process; positive regulation of lipid biosynthetic process; purine nucleotide biosynthetic process; brain development; adenosine metabolic process; response to aluminum ion; AMP catabolic process