Rabbit PDE6A Polyclonal Antibody | anti-PDE6A antibody

Rabbit Polyclonal to Human PDE6A

Cat. Num. AAA240118

• Gene Names: PDE6A; PDEA; RP43; CGPR-A
• Reactivity: Gibbon, Gorilla, Hamster, Human, Monkey, Mouse, Rat; Predicted Reactivity: Dog, Rabbit (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: PDE6A; Polyclonal Antibody; Rabbit Polyclonal to Human PDE6A; Anti-PDE6A Antibody (N-Terminus) IHC-plus; Alpha-PDE; CGMP phosphodiesterase alpha; CGPR-A; PDE6alpha; PDE V-B1; PDEA; Phosphodiesterase 6a; RP43; GMP-PDE alpha; Human PDE6A; anti-PDE6A antibody

• Host: Rabbit
• Reactivity: Gibbon, Gorilla, Hamster, Human, Monkey, Mouse, Rat; Predicted Reactivity: Dog, Rabbit (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human PDE6A / PDE6 Alpha. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.1% sodium azide.
• Concentration: 0.23 mg/ml (varies by lot)
• Sequence Length: 860

• Applicable Applications for anti-PDE6A antibody: Immunohistochemistry (IHC - Paraffin)
• Application Notes: IHC-P (10 - 15 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Human
• Immunogen Description: Synthetic 18 amino acid peptide from N-terminus of human PDE6A / PDE6 Alpha. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster (100%); Dog, Panda, Rabbit (94%).
• Immunogen Type: Synthetic peptide
• Immunogen: PDE6A antibody was raised against synthetic 18 amino acid peptide from N-terminus of human PDE6A / PDE6 Alpha. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster (100%); Dog, Panda, Rabbit (94%); Bovine, Bat, Elephant (89%); Horse, Opossum (83%).
• Antigen Modification: N-Terminus
• Preparation and Storage: Long term: -70 degree C; Short term: +4 degree C

Immunohistochemistry (IHC)

(Anti-PDE6A / PDE6 Alpha antibody IHC of human eye, retina. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Related Product Information for anti-PDE6A antibody

Phosphodiesterase 6A, a catalytic subunit of the photoreceptor phosphodiesterase, has been shown to affect functioning of eye retina as the alpha subunit of the heterotetrameric rod photoreceptor. Mutations in PDE6A have been identified as a cause of autosomal recessive retinitis pigmentosa.

Product Categories/Family for anti-PDE6A antibody

Family: Phosphodiesterase; Subfamily: PDE - photoreceptor

NCBI and Uniprot Product Information

• NCBI GI #: 170650674
• NCBI GeneID: 5145
• NCBI Accession #: NP_000431.2
• NCBI GenBank Nucleotide #: NM_000440.2
• UniProt Accession #: P16499; Q0P638
• Molecular Weight: 99,547 Da
• NCBI Official Full Name: rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha
• NCBI Official Synonym Full Names: phosphodiesterase 6A, cGMP-specific, rod, alpha
• NCBI Official Symbol: PDE6A
• NCBI Official Synonym Symbols: PDEA; RP43; CGPR-A
• NCBI Protein Information: rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha; PDE V-B1; GMP-PDE alpha; cGMP phosphodiesterase alpha subunit; rod photoreceptor cGMP phosphodiesterase alpha subunit
• UniProt Protein Name: Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha
• Protein Family: Rod cGMP-specific 3',5'-cyclic phosphodiesterase
• UniProt Gene Name: PDE6A
• UniProt Synonym Gene Names: PDEA; GMP-PDE alpha
• UniProt Entry Name: PDE6A_HUMAN

NCBI Description

This gene encodes the cyclic-GMP (cGMP)-specific phosphodiesterase 6A alpha subunit, expressed in cells of the retinal rod outer segment. The phosphodiesterase 6 holoenzyme is a heterotrimer composed of an alpha, beta, and two gamma subunits. cGMP is an important regulator of rod cell membrane current, and its dynamic concentration is established by phosphodiesterase 6A cGMP hydrolysis and guanylate cyclase cGMP synthesis. The protein is a subunit of a key phototransduction enzyme and participates in processes of transmission and amplification of the visual signal. Mutations in this gene have been identified as one cause of autosomal recessive retinitis pigmentosa. [provided by RefSeq, Jul 2008]

Uniprot Description

PDE6A: This protein participates in processes of transmission and amplification of the visual signal. Defects in PDE6A are the cause of retinitis pigmentosa type 43 (RP43). RP43 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. Belongs to the cyclic nucleotide phosphodiesterase family.

Protein type: Phosphodiesterase; EC 3.1.4.35

Chromosomal Location of Human Ortholog: 5q31.2-q34

Cellular Component: plasma membrane

Molecular Function: 3',5'-cyclic-GMP phosphodiesterase activity; metal ion binding

Biological Process: rhodopsin mediated signaling; phototransduction, visible light; cytosolic calcium ion homeostasis; regulation of rhodopsin mediated signaling; visual perception; retina development in camera-type eye; GMP metabolic process

Disease: Retinitis Pigmentosa 43