Rabbit anti-Human PDCD10 Polyclonal Antibody | anti-PDCD10 antibody

Anti-PDCD10 Antibody, Rabbit Polyclonal

Cat. Num. AAA8108895

• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: PDCD10; Polyclonal Antibody; Anti-PDCD10 Antibody; Rabbit Polyclonal; PDCD10 Antibody; Rabbit PAb; Antigen Affinity Purified; programmed cell death 10; Anti-CCM3 Antibody; Anti-TFAR15 Antibody; anti-PDCD10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human PDCD10
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-PDCD10 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IP: 5-10uL/mg of lysate
• Immunogen: E Coli-derived Human PDCD10 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human PDCD10 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-PDCD10 rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lane C: K562 Whole Cell Lysate Lane D: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:25 kDa Observed band size:27 kDa)

Immunoprecipitation (IP)

(PDCD10 was immunoprecipitated using: Lane A:0.5 mg HeLa Whole Cell Lysate 4 uL anti-PDCD10 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PDCD10 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 25 kDa Observed band size :28 kDa)

Related Product Information for anti-PDCD10 antibody

PDCD10 (programmed cell death 10, TFAR15), a novel protein associated with cell apoptosis has been recently implicated in mutations associated with Cerebral Cavernous Malformations (CCM). PDCD10 modulation of ERK signaling was mediated by MST4, and PDCD10 could be a regulatory adaptor necessary for MST4 function, suggesting a link between cerebral cavernous malformation pathogenesis and the ERK-MAPK cascade via PDCD10/MST4. Mutations in the programmed cell death 10 gene, PDCD10, cause the autosomal-dominant familial cerebral cavernous malformation 3 (CCM3). PDCD10 is a ubiquitous protein expressed in nearly all tissues and various types of cells. It is essential for vascular development and post-natal vessel maturation, and, has shown a dual function in different types of cancers and chemo-resistance.

References

You C, et al. (2017) Ephb4 forward signalling mediates angiogenesis caused by ccm3/pdcd10-ablation. J Cell Mol Med 21 (9): 1848-1858.Urfali-Mamatoglu C, et al. (2018) Dual function of programmed cell death 10 (pdcd10) in drug resistance. Biomed Pharmacother 101 129-136.Ma X, et al. (2007) Pdcd10 interacts with ste20-related kinase mst4 to promote cell growth and transformation via modulation of the erk pathway. Mol Biol Cell 18 (6): 1965-1978.