Rabbit anti-Human PCSK5 Polyclonal Antibody | anti-PCSK5 antibody

PCSK5, NT (PCSK5, PC5, PC6, Proprotein convertase subtilisin/kexin type 5, Proprotein convertase 5, Proprotein convertase 6, Subtilisin/kexin-like protease PC5) (AP)

Cat. Num. AAA6331858

• Gene Names: PCSK5; PC5; PC6; PC6A; SPC6
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PCSK5; Polyclonal Antibody; NT (PCSK5; PC5; PC6; Proprotein convertase subtilisin/kexin type 5; Proprotein convertase 5; Proprotein convertase 6; Subtilisin/kexin-like protease PC5) (AP); Subtilisin/kexin-like protease PC5; anti-PCSK5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-PCSK5 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PCSK5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-124 amino acids from the N-terminal region of human PCSK5.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PCSK5 Antibody (N-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the PCSK5 antibody detected the PCSK5 protein (arrow).)

Related Product Information for anti-PCSK5 antibody

The protein encoded by this gene belongs to the subtilisin-like proprotein convertase family. The members of this family are proprotein convertases that process latent precursor proteins into their biologically active products. This encoded protein mediates posttranslational endoproteolytic processing for several integrin alpha subunits. It is thought to process prorenin, pro-membrane type-1 matrix metalloproteinase and HIV-1 glycoprotein gp160. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-PCSK5 antibody

Antibodies; Enzymes; Protease (Proteinase; Peptidase)

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Heng, S., et al. Endocrinology 151(8):3909-3917(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Iatan, I., et al. Circ Cardiovasc Genet 2(5):467-475(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 20336246
• NCBI GeneID: 5125
• NCBI Accession #: NP_006191.2
• NCBI GenBank Nucleotide #: NM_006200.5
• UniProt Accession #: Q92824; Q13527; Q96EP4; F5H2G7
• Molecular Weight: 101,649 Da
• NCBI Official Full Name: proprotein convertase subtilisin/kexin type 5 isoform PC6A preproprotein
• NCBI Official Synonym Full Names: proprotein convertase subtilisin/kexin type 5
• NCBI Official Symbol: PCSK5
• NCBI Official Synonym Symbols: PC5; PC6; PC6A; SPC6
• NCBI Protein Information: proprotein convertase subtilisin/kexin type 5
• UniProt Protein Name: Proprotein convertase subtilisin/kexin type 5
• Protein Family: Proprotein convertase subtilisin/kexin
• UniProt Gene Name: PCSK5
• UniProt Synonym Gene Names: PC5; PC6; PC5; PC6; hPC6

NCBI Description

This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an initial autocatalytic processing event in the ER to generate a heterodimer which exits the ER. It then sorts to the trans-Golgi network where a second autocatalytic event takes place and the catalytic activity is acquired. This encoded protein is widely expressed and one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. It mediates posttranslational endoproteolytic processing for several integrin alpha subunits and is thought to process prorenin, pro-membrane type-1 matrix metalloproteinase and HIV-1 glycoprotein gp160. Alternative splicing results in multiple transcript variants, some of which encode distinct isoforms, including a protease packaged into dense core granules (PC5A) and a type 1 membrane bound protease (PC5B). [provided by RefSeq, May 2014]

Uniprot Description

Serine endoprotease that processes various proproteins by cleavage at paired basic amino acids, recognizing the RXXX[KR]R consensus motif. Likely functions in the constitutive and regulated secretory pathways. Plays an essential role in pregnancy establishment by proteolytic activation of a number of important factors such as BMP2, CALD1 and alpha-integrins.