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Western Blot (WB) (Western Blot analysis of extracts of Mouse pancreas, using PTGER1 antibody at 1:1000 dilution.)

Rabbit anti-Mouse, Rat PTGER1 Polyclonal Antibody | anti-PTGER1 antibody

PTGER1 Polyclonal Antibody

Gene Names
PTGER1; EP1
Reactivity
Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
PTGER1; Polyclonal Antibody; PTGER1 Polyclonal Antibody; Prostaglandin E2 receptor EP1 subtype; PGE receptor EP1 subtype; PGE2 receptor EP1 subtype; Prostanoid EP1 receptor; anti-PTGER1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
PTGER1 polyclonal antibody detects endogenous levels of PTGER1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
402
Applicable Applications for anti-PTGER1 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
Recombinant full length Human PTGER1.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of Mouse pancreas, using PTGER1 antibody at 1:1000 dilution.)

Western Blot (WB) (Western Blot analysis of extracts of Mouse pancreas, using PTGER1 antibody at 1:1000 dilution.)
Related Product Information for anti-PTGER1 antibody
Prostaglandin E2, a member of the autacoid family of lipid mediators, is a major renal cyclooxygenase product of arachidonic acid metabolism (1-3). Prostaglandin E2 binds to four G protein-coupled E-prostanoid receptors, designated EP1, EP2, EP3 and EP4 (1-3). The expression and function of the prostaglandin E2 receptors have been highly characterized in kidney (2, 3). EP1, which is predominantly expressed in the collecting duct, couples to Gq proteins to inhibit sodium absorption and increase in intracellular calcium, which act as second messengers. EP2 is coupled to Gs proteins, which stimulate adenylyl cyclase. EP2 has the lowest expression in kidney, but EP2 knockout mice exhibit salt-sensitive hypertension, which suggests a role for EP2 in salt excretion. EP3 is expressed in renal vessels, thick ascending limb and collecting duct. EP3 has at least 6 alternative splice variants that couple to Gi proteins to inhibit cAMP, which subsequently inhibit sodium and water transport. In uterus, EP3 induces the contraction of uterine smooth muscles. EP4 is expressed in glomerulus and collecting duct. It couples to Gs proteins, which stimulate adenylyl cyclase and regulate glomerular tone and renal renin release.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
~ 42kDa
NCBI Official Full Name
Prostaglandin E2 receptor EP1 subtype
NCBI Official Synonym Full Names
prostaglandin E receptor 1
NCBI Official Symbol
PTGER1
NCBI Official Synonym Symbols
EP1
NCBI Protein Information
prostaglandin E2 receptor EP1 subtype
UniProt Protein Name
Prostaglandin E2 receptor EP1 subtype
Protein Family
UniProt Gene Name
PTGER1
UniProt Synonym Gene Names
PGE receptor EP1 subtype; PGE2 receptor EP1 subtype
UniProt Entry Name
PE2R1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the G protein-coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). Through a phosphatidylinositol-calcium second messenger system, G-Q proteins mediate this receptor's activity. Knockout studies in mice suggested a role of this receptor in mediating algesia and in regulation of blood pressure. Studies in mice also suggested that this gene may mediate adrenocorticotropic hormone response to bacterial endotoxin. [provided by RefSeq, Jul 2008]

Uniprot Description

PTGER1: Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(q) proteins which activate a phosphatidylinositol-calcium second messenger system. May play a role as an important modulator of renal function. Implicated the smooth muscle contractile response to PGE2 in various tissues. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: prostaglandin E receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; response to lipopolysaccharide

Research Articles on PTGER1

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Product Notes

The PTGER1 ptger1 (Catalog #AAA3011457) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PTGER1 Polyclonal Antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PTGER1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the PTGER1 ptger1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PTGER1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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