Rabbit PCNA Polyclonal Antibody | anti-PCNA antibody

PCNA Antibody

Cat. Num. AAA9405716

• Gene Names: PCNA; ATLD2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: PCNA; Polyclonal Antibody; PCNA Antibody; cyclin; proliferating cell nuclear antigen; anti-PCNA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total PCNA protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 261

• Applicable Applications for anti-PCNA antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500~1:3000; Immunohistochemistry: 1:50~1:100
• Immunogen Type: Peptide
• Immunogen Description: Synthesized peptide derived from human PCNA.
• Target Name: PCNA
• Preparation and Storage: Store at -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PCNA antibody.)

Western Blot (WB)

(Western blot analysis of extracts from HepG2 cells, using PCNA antibody.)

Western Blot (WB)

(Western blot analysis of extracts from Hela cells (Lane 2), colo cells(Lane 3), HepG2 cells (Lane 4) and 293 cells (Lane 5) using PCNA antiobdy. The lane on the left is treated with synthesized peptide.)

Related Product Information for anti-PCNA antibody

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.Anje Sporbert, Nucleic Acids Res., Jun 2005; 33: 3521 - 3528. Sean C. Gehen, Am J Physiol Lung Cell Mol Physiol, Mar 2007; 292: L716 - L724. Basavaraj Bagewadi, J. Virol., Nov 2004; 78: 11890 - 11903. T Iida, I Suetake, Genes Cells, Oct 2002; 7: 997 -

Product Categories/Family for anti-PCNA antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4505641
• NCBI GeneID: 5111
• NCBI Accession #: NP_002583.1
• NCBI GenBank Nucleotide #: NM_002592.2
• UniProt Accession #: P12004; B2R897; D3DW02
• Molecular Weight: 28,769 Da
• NCBI Official Full Name: proliferating cell nuclear antigen
• NCBI Official Synonym Full Names: proliferating cell nuclear antigen
• NCBI Official Symbol: PCNA
• NCBI Official Synonym Symbols: ATLD2
• NCBI Protein Information: proliferating cell nuclear antigen
• UniProt Protein Name: Proliferating cell nuclear antigen
• Protein Family: PCNA-associated factor
• UniProt Gene Name: PCNA
• UniProt Synonym Gene Names: PCNA
• UniProt Entry Name: PCNA_HUMAN

NCBI Description

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]

Uniprot Description

PCNA: This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Homotrimer. Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with EXO1, POLH, POLK, DNMT1, ERCC5, FEN1, CDC6 and POLDIP2. Interacts with APEX2; this interaction is triggered by reactive oxygen species and increased by misincorporation of uracil in nuclear DNA. Forms a ternary complex with DNTTIP2 and core histone. Interacts with KCTD10 and PPP1R15A. Interacts with POLD1, POLD3 and POLD4. Interacts with BAZ1B; the interaction is direct. Interacts with HLTF and SHPRH. Interacts with NUDT15. Interaction is disrupted in response to UV irradiation and acetylation. Interacts with p21Cip1/p21(CIP1) and CDT1; interacts via their PIP-box which also recruits the DCX(DTL) complex. Interacts with DDX11. Interacts with EGFR; positively regulates PCNA. Interacts with C12orf48/PARI. Interacts with SMARCAD1. Belongs to the PCNA family.

Protein type: Cell cycle regulation; DNA replication

Chromosomal Location of Human Ortholog: 20pter-p12

Cellular Component: nucleoplasm; centrosome; PCNA complex; nuclear replication fork; cytoplasm; DNA replication factor C complex; nucleus

Molecular Function: identical protein binding; protein binding; DNA polymerase processivity factor activity; MutLalpha complex binding; purine-specific mismatch base pair DNA N-glycosylase activity; receptor tyrosine kinase binding; dinucleotide insertion or deletion binding

Biological Process: mismatch repair; heart development; positive regulation of deoxyribonuclease activity; DNA strand elongation during DNA replication; DNA repair; leading strand elongation; response to lipid; telomere maintenance via semi-conservative replication; G1/S-specific transcription in mitotic cell cycle; cell proliferation; bypass DNA synthesis; response to cadmium ion; epithelial cell differentiation; base-excision repair; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; mitotic cell cycle; nucleotide-excision repair, DNA gap filling; telomere maintenance; regulation of DNA replication; G1/S transition of mitotic cell cycle

Disease: Ataxia-telangiectasia-like Disorder 2