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Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human placenta tissue, using CYB5R3 Antibody. The picture on the right is blocked with the synthesized peptide.)

Rabbit anti-Human CYB5R3 Polyclonal Antibody | anti-CYB5R3 antibody

CYB5R3 Antibody

Gene Names
CYB5R3; B5R; DIA1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Synonyms
CYB5R3; Polyclonal Antibody; CYB5R3 Antibody; NADH-cytochrome b5 reductase 3; Cytochrome b5 reductase; B5R; Diaphorase-1; anti-CYB5R3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
CYB5R3 Antibody detects endogenous levels of total CYB5R3 protein.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
334
Applicable Applications for anti-CYB5R3 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:40000
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human CYB5R3.
Immunogen Range
137-186
Preparation and Storage
Stable at -20 degree C for at least 1 year.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human placenta tissue, using CYB5R3 Antibody. The picture on the right is blocked with the synthesized peptide.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human placenta tissue, using CYB5R3 Antibody. The picture on the right is blocked with the synthesized peptide.)

Western Blot (WB)

(Western blot analysis of lysates from HepG2, COLO, HUVEC, HT-29, and K562 cells, using CYB5R3 Antibody. The lane on the right is blocked with the synthesized peptide.)

Western Blot (WB) (Western blot analysis of lysates from HepG2, COLO, HUVEC, HT-29, and K562 cells, using CYB5R3 Antibody. The lane on the right is blocked with the synthesized peptide.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
34 kDa
NCBI Official Full Name
NADH-cytochrome b5 reductase 3 isoform 1
NCBI Official Synonym Full Names
cytochrome b5 reductase 3
NCBI Official Symbol
CYB5R3
NCBI Official Synonym Symbols
B5R; DIA1
NCBI Protein Information
NADH-cytochrome b5 reductase 3
UniProt Protein Name
NADH-cytochrome b5 reductase 3
UniProt Gene Name
CYB5R3
UniProt Synonym Gene Names
DIA1; B5R; Cytochrome b5 reductase

NCBI Description

This gene encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. Alternate splicing results in multiple transcript variants. Mutations in this gene cause methemoglobinemias. [provided by RefSeq, Jan 2010]

Uniprot Description

Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.

Research Articles on CYB5R3

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Product Notes

The CYB5R3 cyb5r3 (Catalog #AAA9506572) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CYB5R3 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CYB5R3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000. Researchers should empirically determine the suitability of the CYB5R3 cyb5r3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CYB5R3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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