Mouse Smooth Muscle Myosin Heavy Chain (SM-MHC) Monoclonal Antibody | anti-MYH11 antibody

Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) Mouse Monoclonal Antibody

Cat. Num. AAA439430

• Gene Names: MYH11; AAT4; FAA4; SMHC; SMMHC
• Reactivity: Human, Cow, Pig, Dog, Cat, Rabbit, Rat, Guinea Pig, Chicken
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Synonyms: Smooth Muscle Myosin Heavy Chain (SM-MHC); Monoclonal Antibody; Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) Mouse Monoclonal Antibody; AAT4; Myosin heavy chain 11 (MYH11); Myosin heavy chain 11 smooth muscle; Smooth muscle myosin heavy chain 11; Myosin-11; SM1; SM2; SMHC; SMMHC; anti-MYH11 antibody

• Host: Mouse
• Reactivity: Human, Cow, Pig, Dog, Cat, Rabbit, Rat, Guinea Pig, Chicken
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: ID8
• Specificity: Smooth muscle myosin heavy chain (SM-MHC) is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmentally regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2. The antibody may be useful for the study of breast tumors as the presence of an intact layer of myoepithelial cells is an important feature, which may distinguish benign breast lesions and carcinoma in situ from invasive tumors.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 1945

• Applicable Applications for anti-MYH11 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
• Application Notes: Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilutions for a specific application should be determined.
• Cellular Localization: Cytoplasmic
• Immunogen: Human uterus extract
• Hu-Chromosome Location: 16p13.11
• Positive Control: Uterus or normal breast
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Non-hazardous.; No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with SM-MHC Monoclonal Antibody (ID8).)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with SM-MHC Monoclonal Antibody (ID8).)

References

D.Lazard,.X. Sastre, M.G.Frid, M.A.Glukhova, J.-P. Thiery and V.E.Koteliansky. Expression of smooth muscle-specific proteins in myoepithelium and stromal myofibroblasts of normal and malignant human breast tissue. Proc. Natl. Acad. Sci. USA, 1993, v.90: 999-1003. N.P. Wang, B.C. Wan, M. Skelly, M.G. Frid, M.A. Glukhova, V.E. Koteliansky, A.M. Gown. Antibodies to novel myoepithelium-associated proteins distinguish benign lesions and in-situ- carcinoma from invasive carcinoma of the breast. Applied Immunohistochemistry 1997;5(3):141-151

NCBI and Uniprot Product Information

• NCBI GI #: 92091583
• NCBI GeneID: 4629
• NCBI Accession #: NP_001035202.1
• NCBI GenBank Nucleotide #: NM_001040113.1
• UniProt Accession #: P35749; O00396; O94944; P78422; Q3MIV8; Q3MNF0; Q3MNF1; D2JYH7
• Molecular Weight: 205kDa (MHC-1); 200kDa (MHC-2)
• NCBI Official Full Name: myosin-11 isoform SM2B
• NCBI Official Synonym Full Names: myosin heavy chain 11
• NCBI Official Symbol: MYH11
• NCBI Official Synonym Symbols: AAT4; FAA4; SMHC; SMMHC
• NCBI Protein Information: myosin-11
• UniProt Protein Name: Myosin-11
• Protein Family: Myosin
• UniProt Gene Name: MYH11
• UniProt Synonym Gene Names: KIAA0866
• UniProt Entry Name: MYH11_HUMAN

NCBI Description

The protein encoded by this gene is a smooth muscle myosin belonging to the myosin heavy chain family. The gene product is a subunit of a hexameric protein that consists of two heavy chain subunits and two pairs of non-identical light chain subunits. It functions as a major contractile protein, converting chemical energy into mechanical energy through the hydrolysis of ATP. The gene encoding a human ortholog of rat NUDE1 is transcribed from the reverse strand of this gene, and its 3' end overlaps with that of the latter. The pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript that encodes a protein consisting of the first 165 residues from the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Alternative splicing generates isoforms that are differentially expressed, with ratios changing during muscle cell maturation. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

MYH11: Muscle contraction. A chromosomal aberration involving MYH11 is found in acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein consisting of the 165 N-terminal residues of CBF-beta (PEPB2) and the tail region of MYH11. Defects in MYH11 are the cause of familial aortic aneurysm thoracic type 4 (AAT4); also known as familial thoracic aortic aneurysm and dissection (TAAD). Aneurysms and dissections of the aorta usually result from degenerative changes in the aortic wall. Thoracic aortic aneurysms and dissections are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. Patients with AAT4 show marked aortic stiffness. Pathological aortas show large areas of medial degeneration with very low smooth muscle cells content. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motor; Motility/polarity/chemotaxis; Oncoprotein

Chromosomal Location of Human Ortholog: 16p13.11

Cellular Component: cytosol; muscle myosin complex

Molecular Function: protein binding; structural constituent of muscle

Biological Process: cardiac muscle fiber development; elastic fiber assembly; muscle contraction; muscle thick filament assembly; smooth muscle contraction

Disease: Aortic Aneurysm, Familial Thoracic 4