Rabbit anti-Human, Mouse PBK Polyclonal Antibody | anti-PBK antibody

Rabbit anti-human PBK polyclonal Antibody

Cat. Num. AAA1498241

• Gene Names: PBK; SPK; CT84; TOPK; HEL164; Nori-3
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen Affinity Purified
• Synonyms: PBK; Polyclonal Antibody; Rabbit anti-human PBK polyclonal Antibody; Cancer/testis antigen 84; CT84; MAPKK-like protein kinase; Nori-3; PDZ-binding kinase; Spermatogenesis-related protein kinase; SPK; T-LAK cell-originated protein kinase; TOPK; anti-PBK antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Sequence Length: 333

• Applicable Applications for anti-PBK antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Recommended dilution: WB:1:200-1000; IHC:1:20-1:200
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Conjugate: Non-conjugated
• Immunogen: Recombinant human Lymphokine-activated killer T-cell-originated protein kinase protein (1-322aa)
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-15243-R
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(All lanes: PBK antibody at 6ug/mlLane 1:Hela whole cell lysateLane 2:mouse gonad tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 37,38 kDaObserved band size: 36 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human tonsil using MBS1498241 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded placenta tonsil using MBS1498241 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human thymus using MBS1498241 at dilution 1:100)

Related Product Information for anti-PBK antibody

Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.

NCBI and Uniprot Product Information

• NCBI GI #: 525507092
• NCBI GeneID: 55872
• NCBI Accession #: NP_001265874.1
• NCBI GenBank Nucleotide #: NM_001278945.1
• UniProt Accession #: Q96KB5; Q9NPD9; Q9NYL7; Q9NZK6; B4DX68; D3DST2
• Molecular Weight: 37,211 Da
• NCBI Official Full Name: lymphokine-activated killer T-cell-originated protein kinase isoform 2
• NCBI Official Synonym Full Names: PDZ binding kinase
• NCBI Official Symbol: PBK
• NCBI Official Synonym Symbols: SPK; CT84; TOPK; HEL164; Nori-3
• NCBI Protein Information: lymphokine-activated killer T-cell-originated protein kinase
• UniProt Protein Name: Lymphokine-activated killer T-cell-originated protein kinase
• Protein Family: Lymphokine-activated killer T-cell-originated protein kinase
• UniProt Gene Name: PBK
• UniProt Synonym Gene Names: TOPK; CT84; SPK
• UniProt Entry Name: TOPK_HUMAN

NCBI Description

This gene encodes a serine/threonine protein kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of this gene has been implicated in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

PBK: Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage. Interacts with DLG1 and TP53. Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules. Activated by phosphorylation. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase kinase subfamily.

Protein type: Cancer Testis Antigen (CTA); Protein kinase, Other; Kinase, protein; EC 2.7.12.2; Protein kinase, Ser/Thr (non-receptor); Other group; TOPK family

Chromosomal Location of Human Ortholog: 8p21.2

Cellular Component: nucleus

Molecular Function: ATP binding; protein binding; protein serine/threonine kinase activity

Biological Process: mitosis; negative regulation of inflammatory response; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; negative regulation of stress-activated MAPK cascade; protein amino acid phosphorylation