Mouse anti-Human PBK Monoclonal Antibody | anti-PBK antibody

PBK Antibody

Cat. Num. AAA9602541

• Gene Names: PBK; SPK; CT84; TOPK; HEL164; Nori-3
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA, Flow Cytometry, Functional Assay
• Purity: Affinity-Chromatography
• Synonyms: PBK; Monoclonal Antibody; PBK Antibody; Cancer/testis antigen 84; CT84; Epididymis luminal protein 164; FLJ14385; HEL164; Lymphokine activated killer T cell originated protein kinase; Lymphokine-activated killer T-cell-originated protein kinase; MAPKK like protein kinase; MAPKK-like protein kinase; Nori 3; Nori-3; Nori3; PDZ binding kinase; PDZ-binding kinase; Serine/threonine protein kinase; Spermatogenesis related protein kinase; Spermatogenesis-related protein kinase; SPK; T LAK cell originated protein kinase; T-LAK cell-originated protein kinase; TOPK; TOPK_HUMAN; anti-PBK antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: PBK antibody detects endogenous levels of total PBK
• Purity/Purification: Affinity-Chromatography
• Form/Format: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 333

• Applicable Applications for anti-PBK antibody: Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA), Flow Cytometry (FC/FACS)
• Application Notes: ELISA: 1:10000; WB: 1:500-1:2000; IHC: 1:200-1:1000; ICC: 1:200-1:1000; FC/FACS: 1:200-1:400
• Immunogen: Purified recombinant fragment of human PBK expressed in E. Coli
• Tissue Specificity: Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Figure 2: Western blot analysis using PBK mAb against human PBK (AA: 50-230) recombinant protein. (Expected MW is 45.8 kDa) )

Related Product Information for anti-PBK antibody

Function: Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.
Subunit Structure: Interacts with DLG1 and TP53.
Post-translational Modifications: Phosphorylated; in a cell-cycle dependent manner at mitosis.
Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 525507092
• NCBI GeneID: 55872
• NCBI Accession #: NP_001265874.1
• NCBI GenBank Nucleotide #: NM_001278945.1
• UniProt Accession #: Q96KB5; Q9NPD9; Q9NYL7; Q9NZK6; B4DX68; D3DST2
• Molecular Weight: Observed: 36 kDa; Predicted: 37 kDa
• NCBI Official Full Name: lymphokine-activated killer T-cell-originated protein kinase isoform 2
• NCBI Official Synonym Full Names: PDZ binding kinase
• NCBI Official Symbol: PBK
• NCBI Official Synonym Symbols: SPK; CT84; TOPK; HEL164; Nori-3
• NCBI Protein Information: lymphokine-activated killer T-cell-originated protein kinase
• UniProt Protein Name: Lymphokine-activated killer T-cell-originated protein kinase
• Protein Family: Lymphokine-activated killer T-cell-originated protein kinase
• UniProt Gene Name: PBK
• UniProt Synonym Gene Names: TOPK; CT84; SPK

NCBI Description

This gene encodes a serine/threonine protein kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of this gene has been implicated in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.