Parvin alpha/PARVA Recombinant Protein | PARVA recombinant protein

Parvin alpha/PARVA Protein, Human, Recombinant (GST Tag)

Cat. Num. AAA8121583

• Gene Names: PARVA; MXRA2; CH-ILKBP
• Purity: >60% as determined by SDS-PAGE
• Synonyms: Parvin alpha/PARVA; Parvin alpha/PARVA Protein; Human; Recombinant (GST Tag); Human PARVA/Actopaxin Protein (GST Tag); CH-ILKBP Protein; MXRA2 Protein; parvin; alpha; PARVA recombinant protein

• Host: E Coli
• Purity/Purification: >60% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Glu372

• Species: Human
• Predicted N Terminal: Met
• Tag: N-GST
• Protein Construction: A DNA sequence encoding the mature form of human PARVA (Q9NVD7-1)(Met1-Glu372) was fused with the GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PARVA recombinant protein

Background: Actopaxin, also known as alpha-parvin, belongs to the parvin family. It is widely expressed, with highest levels in heart, skeletal muscle, kidney and liver. Actopaxin contains 2 CH (calponin-homology) domains and probably plays a role in the regulation of cell adhesion and cytoskeleton organization. It interacts with integrin-linked protein kinase and probably with actin and the LD1 and LD4 motifs of PXN. Actopaxin binds directly to both F-actin and paxillin LD1 and LD4 motifs. Actopaxin also exhibits robust focal adhesion localization in several cultured cell types but is not found along the length of the associated actin-rich stress fibers. It is absent from actin-rich cell-cell adherens junctions.

References

Korenbaum E, et al. (2002) Genomic organization and expression profile of the parvin family of focal adhesion proteins in mice and humans. Gene. 279(1):69-79.Nikolopoulos SN, et al. (2002) Molecular dissection of actopaxin-integrin-linked kinase-Paxillin interactions and their role in subcellular localization. J Biol Chem. 277(2): 1568-75.Tu Y, et al. (2001) A new focal adhesion protein that interacts with integrin-linked kinase and regulates cell adhesion and spreading. J Cell Biol. 153(3): 585-98.

NCBI and Uniprot Product Information

• NCBI GI #: 20139236
• NCBI GeneID: 55742
• UniProt Accession #: Q9NVD7; Q96C85; Q9HA48
• Molecular Weight: 372
• NCBI Official Full Name: Alpha-parvin
• NCBI Official Synonym Full Names: parvin, alpha
• NCBI Official Symbol: PARVA
• NCBI Official Synonym Symbols: MXRA2; CH-ILKBP
• NCBI Protein Information: alpha-parvin; actopaxin; matrix-remodeling-associated protein 2; calponin-like integrin-linked kinase-binding protein
• UniProt Protein Name: Alpha-parvin
• Protein Family: Alpha-parvin
• UniProt Gene Name: PARVA
• UniProt Synonym Gene Names: MXRA2
• UniProt Entry Name: PARVA_HUMAN

NCBI Description

This gene encodes a member of the parvin family of actin-binding proteins. Parvins are associated with focal contacts and contain calponin homology domains that bind to actin filaments. The encoded protein is part of the integrin-linked kinase signaling complex and plays a role in cell adhesion, motility and survival. [provided by RefSeq, Dec 2010]

Uniprot Description

PARVA: Probably plays a role in the regulation of cell adhesion and cytoskeleton organization. Plays a role in ciliogenesis. Interacts with TGFB1I1. Interacts with integrin-linked protein kinase and probably with actin and the LD1 and LD4 motifs of PXN. Interacts with ARHGAP31. Widely expressed, with highest levels in heart, skeletal muscle, kidney and liver. Belongs to the parvin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 11p15.3

Cellular Component: focal adhesion; lamellipodium; cytoplasm; plasma membrane; nucleus; Z disc; cytosol; actin cytoskeleton

Molecular Function: protein binding; actin binding

Biological Process: heterotypic cell-cell adhesion; regulation of cell shape; actin cytoskeleton reorganization; establishment and/or maintenance of cell polarity; sprouting angiogenesis