Western Blot (WB)
(Parp12 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the Parp12 antibody detected the Parp12 protein (arrow).)
Rabbit anti-Human Parp12 Polyclonal Antibody | anti-PARP12 antibody
Parp12, NT (Poly [ADP-ribose] Polymerase 12, PARP-12, Zinc Finger CCCH Domain-containing Protein 1, ZC3HDC1)
Gene Names
PARP12; ZC3H1; ARTD12; MST109; MSTP109; ZC3HDC1
Reactivity
Human
Applications
ELISA, Immunohistochemistry
Purity
PurifiedPurified by ammonium sulfate precipitation.
Synonyms
Parp12; Polyclonal Antibody; NT (Poly [ADP-ribose] Polymerase 12; PARP-12; Zinc Finger CCCH Domain-containing Protein 1; ZC3HDC1); Anti -Parp12; anti-PARP12 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human NFATC2.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Purified by ammonium sulfate precipitation.
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Applicable Applications for anti-PARP12 antibody
ELISA (EL/EIA), Immunohistochemistry (IHC)
Application Notes
Suitable for use in ELISA and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Immunohistochemistry: 1:10-1:50
Dilution: ELISA: 1:1,000
Immunohistochemistry: 1:10-1:50
Immunogen
Synthetic peptide selected from the N-terminal region of human Parp12 (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Parp12 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the Parp12 antibody detected the Parp12 protein (arrow).)
Western Blot (WB)
(Parp12 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the Parp12 antibody detected the Parp12 protein (arrow).)
Immunohistochemistry (IHC)
(Parp12 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Parp12 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Parp12 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Parp12 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(Parp12 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Parp12 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-PARP12 antibody
Poly(ADP-ribosyl)ation is an immediate DNA-damage-dependent post-translational modification of histones and other nuclear proteins that contributes to the survival of injured proliferating cells. Poly(ADP-ribose) polymerases (PARPs) now constitute a large family of 18 proteins, encoded by different genes and displaying a conserved catalytic domain in which PARP-1 (113kD), the founding member, and PARP-2 (62kD) are so far the sole enzymes whose catalytic activity has been shown to be immediately stimulated by DNA strand breaks. A large repertoire of sequences encoding novel PARPs now extends considerably the field of poly(ADP-ribosyl)ation reactions to various aspects of the cell biology including cell proliferation and cell death. Some of these new members interact with each other, share common partners and common subcellular localizations suggesting possible fine tuning in the regulation of this post-translational modification of proteins.
Product Categories/Family for anti-PARP12 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
79,064 Da
NCBI Official Full Name
poly
NCBI Official Synonym Full Names
poly (ADP-ribose) polymerase family, member 12
NCBI Official Symbol
PARP12
NCBI Official Synonym Symbols
ZC3H1; ARTD12; MST109; MSTP109; ZC3HDC1
NCBI Protein Information
poly [ADP-ribose] polymerase 12; poly [ADP-ribose] polymerase 12; zinc finger CCCH type domain containing 1; zinc finger CCCH domain-containing protein 1; ADP-ribosyltransferase diphtheria toxin-like 12
UniProt Protein Name
Poly [ADP-ribose] polymerase 12
Protein Family
UniProt Gene Name
PARP12
UniProt Synonym Gene Names
ZC3HDC1; PARP-12; ARTD12
UniProt Entry Name
PAR12_HUMAN
Uniprot Description
PARP12:
Protein type: Transferase; EC 2.4.2.30
Chromosomal Location of Human Ortholog: 7q34
Cellular Component: nucleus
Molecular Function: metal ion binding; NAD+ ADP-ribosyltransferase activity
Biological Process: metabolic process
Research Articles on PARP12
Similar Products
Product Notes
The PARP12 parp12 (Catalog #AAA626385) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Parp12, NT (Poly [ADP-ribose] Polymerase 12, PARP-12, Zinc Finger CCCH Domain-containing Protein 1, ZC3HDC1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Parp12 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Immunohistochemistry (IHC). Suitable for use in ELISA and Immunohistochemistry. Dilution: ELISA: 1:1,000 Immunohistochemistry: 1:10-1:50. Researchers should empirically determine the suitability of the PARP12 parp12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Parp12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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