Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Phosphoglycolate phosphatase (PGP) Standard Curve.)
Mouse Phosphoglycolate Phosphatase (PGP) ELISA Kit | PGP elisa kit
Mouse Phosphoglycolate Phosphatase (PGP) ELISA Kit
Gene Names
Pgp; AUM; G3PP; AI481330; 1700012G19Rik
Reactivity
Mouse
Synonyms
Phosphoglycolate Phosphatase (PGP); Mouse Phosphoglycolate Phosphatase (PGP) ELISA Kit; PGP; MGC4692; PGP elisa kit
Reactivity
Mouse
Specificity
Mouse Phosphoglycolate phosphatase (PGP) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PGP. No significant cross-reactivity or interference between Mouse PGP and analogues was observed.
Sequence Length
321
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Phosphoglycolate phosphatase (PGP) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Phosphoglycolate phosphatase (PGP) Standard Curve.)
Related Product Information for PGP elisa kit
Description: This Mouse Phosphoglycolate phosphatase (PGP) ELISA Kit employs a two-site sandwich ELISA to quantitate PGP in samples. An antibody specific for PGP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PGP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PGP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PGP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: P-glycoprotein is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier.
Background: P-glycoprotein is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
glycerol-3-phosphate phosphatase
NCBI Official Synonym Full Names
phosphoglycolate phosphatase
NCBI Official Symbol
Pgp
NCBI Official Synonym Symbols
AUM; G3PP; AI481330; 1700012G19Rik
NCBI Protein Information
glycerol-3-phosphate phosphatase
UniProt Protein Name
Phosphoglycolate phosphatase
Protein Family
UniProt Gene Name
Pgp
UniProt Synonym Gene Names
PGP; PGPase; AUM
UniProt Entry Name
PGP_MOUSE
Research Articles on PGP
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Product Notes
The Mouse PGP pgp (Catalog #AAA9717367) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717367 ELISA Kit recognizes Mouse PGP. It is sometimes possible for the material contained within the vial of "Phosphoglycolate Phosphatase (PGP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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