Goat PARP12 Polyclonal Antibody | anti-PARP12 antibody
Goat anti-PARP12 Antibody
Gene Names
PARP12; ZC3H1; ARTD12; MST109; MSTP109; ZC3HDC1
Reactivity
Tested: Human; Expected from sequence similarity: Human, Dog
Applications
ELISA, Immunofluorescence
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
PARP12; Polyclonal Antibody; Goat anti-PARP12 Antibody; poly (ADP-ribose) polymerase family; member 12; HGNC: 21919; FLJ22693; PARP-12; ZC3H1; ZC3HDC1; zinc finger CCCH type domain containing 1; zinc finger CCCH-type domain containing 1; PARP12 antibody; member 12 antibody; HGNC: 21919 antibody; FLJ22693 antibody; PARP-12 antibody; ZC3H1 antibody; ZC3HDC1 antibody; zinc finger CCCH type domain containing 1 antibody; zinc finger CCCH-type domain containing 1 antibody; anti-PARP12 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Dog
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
NAHDIKNKSSAP
Sequence Length
701
Applicable Applications for anti-PARP12 antibody
Peptide ELISA (EIA), Immunofluorescence (IF)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Preliminary experiments gave an approx 110kDa band in Human Liver lysates and in lysates of hepatoblastoma cell line HEPG2 after 0. 03ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 79.1kDa according to NP_073587.1. The 110kDa band was successfully blocked by incubation with the immunizing peptide.
Immunofluorescence: Anonymous customer has confirmed specificity by siRNA-mediated PARP2 knockdown.
Western Blot: Preliminary experiments gave an approx 110kDa band in Human Liver lysates and in lysates of hepatoblastoma cell line HEPG2 after 0. 03ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 79.1kDa according to NP_073587.1. The 110kDa band was successfully blocked by incubation with the immunizing peptide.
Immunofluorescence: Anonymous customer has confirmed specificity by siRNA-mediated PARP2 knockdown.
Immunogen
Peptide with sequence C-NAHDIKNKSSAP, from the internal region of the protein sequence according to NP_073587.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
79,064 Da
NCBI Official Full Name
poly
NCBI Official Synonym Full Names
poly(ADP-ribose) polymerase family member 12
NCBI Official Symbol
PARP12
NCBI Official Synonym Symbols
ZC3H1; ARTD12; MST109; MSTP109; ZC3HDC1
NCBI Protein Information
poly [ADP-ribose] polymerase 12
UniProt Protein Name
Poly [ADP-ribose] polymerase 12
Protein Family
UniProt Gene Name
PARP12
UniProt Synonym Gene Names
ZC3HDC1; PARP-12; ARTD12
UniProt Entry Name
PAR12_HUMAN
Uniprot Description
PARP12:
Protein type: Transferase; EC 2.4.2.30
Chromosomal Location of Human Ortholog: 7q34
Cellular Component: nucleus
Molecular Function: metal ion binding; NAD+ ADP-ribosyltransferase activity
Biological Process: metabolic process
Research Articles on PARP12
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Product Notes
The PARP12 parp12 (Catalog #AAA423427) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-PARP12 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's PARP12 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunofluorescence (IF). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Preliminary experiments gave an approx 110kDa band in Human Liver lysates and in lysates of hepatoblastoma cell line HEPG2 after 0. 03ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 79.1kDa according to NP_073587.1. The 110kDa band was successfully blocked by incubation with the immunizing peptide. Immunofluorescence: Anonymous customer has confirmed specificity by siRNA-mediated PARP2 knockdown. Researchers should empirically determine the suitability of the PARP12 parp12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: NAHDIKNKSS AP. It is sometimes possible for the material contained within the vial of "PARP12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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