Rabbit anti-Human, Mouse FADS2 Polyclonal Antibody | anti-FADS2 antibody

FADS2, ID (FADS2, Fatty acid desaturase 2, Delta(6) fatty acid desaturase) (Azide free) (HRP)

Cat. Num. AAA6297607

• Gene Names: FADS2; D6D; DES6; TU13; FADSD6; LLCDL2; SLL0262
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FADS2; Polyclonal Antibody; ID (FADS2; Fatty acid desaturase 2; Delta(6) fatty acid desaturase) (Azide free) (HRP); Delta(6) fatty acid desaturase; anti-FADS2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-FADS2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: FADS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-132 amino acids from the Central region of human FADS2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(FADS2 Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the FADS2 antibody detected the FADS2 protein (arrow).)

Flow Cytometry (FC/FACS)

(FADS2 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-FADS2 antibody

The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members FADS1 and FADS2 at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization.

Product Categories/Family for anti-FADS2 antibody

Antibodies; Enzymes

References

Molto-Puigmarti, C., et al. Am. J. Clin. Nutr. (2010). In press: Lattka, E., et al. Curr. Opin. Lipidol. 21(1):64-69(2010). Lattka, E., et al. J. Lipid Res. 51(1):182-191(2010). Glaser, C., et al. Metab. Clin. Exp. (2009). In press: Lattka, E., et al. J Nutrigenet Nutrigenomics 2(3):119-128(2009). Tang, C., et al. J. Lipid Res. 44(4):686-695(2003). Nara, T.Y., et al. Biochem. Biophys. Res. Commun. 296(1):111-117(2002). Marquardt, A., et al. Genomics 66(2):175-183(2000). Cho, H.P., et al. J. Biol. Chem. 274(1):471-477(1999). Stohr, H., et al. Genome Res. 8(1):48-56(1998).

NCBI and Uniprot Product Information

• NCBI GI #: 4758334
• NCBI GeneID: 9415
• NCBI Accession #: NP_004256.1
• NCBI GenBank Nucleotide #: NM_004265.2
• UniProt Accession #: O95864; Q6MZQ7; Q96H07; Q96SV8; Q9H3G3; Q9Y3X4; A8K2M6
• Molecular Weight: 52,259 Da
• NCBI Official Full Name: fatty acid desaturase 2
• NCBI Official Synonym Full Names: fatty acid desaturase 2
• NCBI Official Symbol: FADS2
• NCBI Official Synonym Symbols: D6D; DES6; TU13; FADSD6; LLCDL2; SLL0262
• NCBI Protein Information: fatty acid desaturase 2; OTTHUMP00000224924; OTTHUMP00000224926; OTTHUMP00000224929; OTTHUMP00000224931; delta-6 desaturase; delta-6-desaturase; delta(6) desaturase; delta-6 fatty acid desaturase; delta(6) fatty acid desaturase; linoleoyl-CoA desaturase (
• UniProt Protein Name: Fatty acid desaturase 2
• Protein Family: Fatty acid desaturase
• UniProt Gene Name: FADS2
• UniProt Synonym Gene Names: D6D; Delta(6) desaturase; Delta-6 desaturase

Uniprot Description

Component of a lipid metabolic pathway that catalyzes biosynthesis of highly unsaturated fatty acids (HUFA) from precursor essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3). Catalyzes the first and rate limiting step in this pathway which is the desaturation of LA (18:2n-6) and ALA (18:3n-3) into gamma-linoleic acid (GLA) (18:3n-6) and stearidonic acid (18:4n-3) respectively and other desaturation steps. Highly unsaturated fatty acids (HUFA) play pivotal roles in many biological functions. It catalizes as well the introduction of a cis double bond in palmitate to produce the mono-unsaturated fatty acid sapienate, the most abundant fatty acid in sebum.