Rabbit anti-Human PAPSS1 Polyclonal Antibody | anti-PAPSS1 antibody

PAPSS1 Antibody (C-term A607)

Cat. Num. AAA9201747

• Gene Names: PAPSS1; SK1; PAPSS; ATPSK1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: PAPSS1; Polyclonal Antibody; PAPSS1 Antibody (C-term A607); Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1; PAPS synthase 1; PAPSS 1; Sulfurylase kinase 1; SK 1; SK1; Sulfate adenylyltransferase; ATP-sulfurylase; Sulfate adenylate transferase; SAT; Adenylyl-sulfate kinase; 3'-phosphoadenosine-5'-phosphosulfate synthase; APS kinase; Adenosine-5'-phosphosulfate 3'-phosphotransferase; Adenylylsulfate 3'-phosphotransferase; ATPSK1; PAPSS; anti-PAPSS1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This PAPSS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 592-624 amino acids from the C-terminal region of human PAPSS1.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 592-624
• Sequence Length: 624

• Applicable Applications for anti-PAPSS1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of anti-PAPSS1 Antibody (C-term A607) in A2058 cell line lysates (35ug/lane). PAPSS1(arrow) was detected using the purified Pab.)

Related Product Information for anti-PAPSS1 antibody

Sulfotransferase (SULT) enzymes catalyze the sulfate conjugation of many drugs, xenobiotic compounds, hormones, and neurotransmitters. 3'-phosphoadenosine 5'-phosphosulfate (PAPS) synthase (PAPSS) catalyzes the biosynthesis of PAPS which serves as the universal sulfonate donor compound for all sulfotransferase reactions. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2 (603005). Bifunctional PAPSS1 is comprised of an N-terminal APS kinase domain, and a C-terminal ATP sulfurylase domain. Full-length protein has significantly less APS kinase activity than the N-terminal fragment, suggesting that the C-terminal domain exerts a regulatory role on the N-terminal APS kinase activity. In humans there are two major isoforms: PAPSS1 and PAPSS2. In brain and skin PAPSS1 is the major isoform, whereas in liver, cartilage and adrenal glands PAPSS2 isoform expression dominates. The predicted 623-amino acid protein is 98% identical to mouse PAPS synthase. The N-terminal 268-amino acid region of human PAPS synthase resembles APS kinases from other organisms and contains 3 conserved nucleotide-binding motifs.

Product Categories/Family for anti-PAPSS1 antibody

Cell Biology

References

Biochemistry 43 (14), 4356-4365 (2004)
IUBMB Life 55 (1), 1-11 (2003)
Biochem. J. 365 (PT 2), 497-504 (2002)
Biochem. Biophys. Res. Commun. 268 (2), 437-444 (2000)
FASEB J. 14 (2), 345-354 (2000)

NCBI and Uniprot Product Information

• NCBI GI #: 46094058
• NCBI GeneID: 9061
• NCBI Accession #: NP_005434.4
• NCBI GenBank Nucleotide #: NM_005443.4
• UniProt Accession #: O43252; O43841; O75332; Q96FB1; Q96TF4; Q9P1P9; Q9UE98
• Molecular Weight: 70833
• NCBI Official Full Name: bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1
• NCBI Official Synonym Full Names: 3'-phosphoadenosine 5'-phosphosulfate synthase 1
• NCBI Official Symbol: PAPSS1
• NCBI Official Synonym Symbols: SK1; PAPSS; ATPSK1
• NCBI Protein Information: bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1
• UniProt Protein Name: Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1
• Protein Family: Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase
• UniProt Gene Name: PAPSS1
• UniProt Synonym Gene Names: ATPSK1; PAPSS; PAPS synthase 1; PAPSS 1; SK 1; SK1; SAT
• UniProt Entry Name: PAPS1_HUMAN

NCBI Description

Three-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) is the sulfate donor cosubstrate for all sulfotransferase (SULT) enzymes (Xu et al., 2000 [PubMed 10679223]). SULTs catalyze the sulfate conjugation of many endogenous and exogenous compounds, including drugs and other xenobiotics. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2 (MIM 603005).[supplied by OMIM, Mar 2008]

Uniprot Description

PAPSS1: Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate- activation pathway. Also involved in the biosynthesis of sulfated L-selectin ligands in endothelial cells.

Protein type: Nucleotide Metabolism - purine; EC 2.7.7.4; EC 2.7.1.25; Kinase, other; Energy Metabolism - sulfur; Other Amino Acids Metabolism - selenoamino acid

Chromosomal Location of Human Ortholog: 4q24

Cellular Component: cytosol

Molecular Function: adenylylsulfate kinase activity; ATP binding; sulfate adenylyltransferase (ATP) activity; nucleotidyltransferase activity

Biological Process: sulfate assimilation; glycosaminoglycan metabolic process; xenobiotic metabolic process; carbohydrate metabolic process; pathogenesis; skeletal development; phosphorylation; 3'-phosphoadenosine 5'-phosphosulfate metabolic process; 3'-phosphoadenosine 5'-phosphosulfate biosynthetic process