Mouse heparin-binding EGF-like growth factor (HBEGF) ELISA Kit | HBEGF elisa kit

Mouse heparin-binding EGF-like growth factor (HBEGF) ELISA Kit

Cat. Num. AAA7220748

• Gene Names: HBEGF; DTR; DTS; DTSF; HEGFL
• Reactivity: Mouse
• Synonyms: heparin-binding EGF-like growth factor (HBEGF); Mouse heparin-binding EGF-like growth factor (HBEGF) ELISA Kit; HBEGF elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 mg/L.
• Intended Uses: This Bordetella pertussis IgG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat Bordetella pertussis IgG. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HBEGF elisa kit

Principle of the assay: Bordetella pertussis IgG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Bordetella pertussis IgG antibody and an Bordetella pertussis IgG-HRP conjugate. The assay sample and buffer are incubated together with Bordetella pertussis IgG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Bordetella pertussis IgG concentration since Bordetella pertussis IgG from samples and Bordetella pertussis IgG-HRP conjugate compete for the anti-Bordetella pertussis IgG antibody binding site. Since the number of sites is limited, as more sites are occupied by Bordetella pertussis IgG from the sample, fewer sites are left to bind Bordetella pertussis IgG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Bordetella pertussis IgG concentration in each sample is interpolated from this standard curve.

Product Categories/Family for HBEGF elisa kit

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 183867
• NCBI GeneID: 1839
• Molecular Weight: 23,067 Da
• NCBI Official Full Name: heparin-binding EGF-like growth factor
• NCBI Official Synonym Full Names: heparin-binding EGF-like growth factor
• NCBI Official Symbol: HBEGF
• NCBI Official Synonym Symbols: DTR; DTS; DTSF; HEGFL
• NCBI Protein Information: proheparin-binding EGF-like growth factor; heparin-binding epidermal growth factor; diphtheria toxin receptor (heparin-binding EGF-like growth factor); diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor)
• UniProt Protein Name: Proheparin-binding EGF-like growth factor
• Protein Family: Proheparin-binding EGF-like growth factor
• UniProt Gene Name: HBEGF
• UniProt Synonym Gene Names: DTR; DTS; HEGFL; HB-EGF; HBEGF; DT-R
• UniProt Entry Name: HBEGF_HUMAN

Uniprot Description

HBEGF: Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 5q23

Cellular Component: extracellular space; cell surface; integral to plasma membrane; plasma membrane; extracellular region

Molecular Function: heparin binding; growth factor activity; epidermal growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of keratinocyte migration; muscle development; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; negative regulation of elastin biosynthetic process; positive regulation of smooth muscle cell proliferation; pathogenesis; signal transduction; positive regulation of cell growth; regulation of heart contraction; positive regulation of protein kinase B signaling cascade; positive regulation of cell proliferation; innate immune response; positive regulation of cell migration; wound healing, spreading of epidermal cells