Rabbit anti-Human PAPSS1 Polyclonal Antibody | anti-PAPSS1 antibody

PAPSS1, CT (A607) (Bifunctional 3'-phosphoadenosine 5'-phosphosulfate Synthethase 1, PAPS Synthethase 1, Sulfurylase Kinase 1, SK1, ATPSK1, PAPSS)

Cat. Num. AAA624414

• Gene Names: PAPSS1; SK1; PAPSS; ATPSK1
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: PAPSS1; Polyclonal Antibody; CT (A607) (Bifunctional 3'-phosphoadenosine 5'-phosphosulfate Synthethase 1; PAPS Synthethase 1; Sulfurylase Kinase 1; SK1; ATPSK1; PAPSS); Anti -PAPSS1; anti-PAPSS1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PAPSS1.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-PAPSS1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500
• Immunogen: Synthetic peptide selected from the C-terminal region of human PAPSS1 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-PAPSS1 Antibody (C-term A607) in A2058 cell line lysates (35ug/lane). PAPSS1(arrow) was detected using the purified Pab.)

Related Product Information for anti-PAPSS1 antibody

Sulfotransferase (SULT) enzymes catalyze the sulfate conjugation of many drugs, xenobiotic compounds, hormones, and neurotransmitters. 3'-phosphoadenosine 5'-phosphosulfate (PAPS) synthase (PAPSS) catalyzes the biosynthesis of PAPS which serves as the universal sulfonate donor compound for all sulfotransferase reactions. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2 (603005). Bifunctional PAPSS1 is comprised of an N-terminal APS kinase domain, and a C-terminal ATP sulfurylase domain. Full-length protein has significantly less APS kinase activity than the N-terminal fragment, suggesting that the C-terminal domain exerts a regulatory role on the N-terminal APS kinase activity. In humans there are two major isoforms: PAPSS1 and PAPSS2. In brain and skin PAPSS1 is the major isoform, whereas in liver, cartilage and adrenal glands PAPSS2 isoform expression dominates. The predicted 623aa protein is 98% identical to mouse PAPS synthase. The N-terminal 268aa region of human PAPS synthase resembles APS kinases from other organisms and contains 3 conserved nucleotide-binding motifs.

Product Categories/Family for anti-PAPSS1 antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 48146173
• NCBI GeneID: 9061
• UniProt Accession #: Q6IAX6; O43252; Q05BW9; Q4W5F0; Q4W5H3
• Molecular Weight: 70,833 Da
• NCBI Official Full Name: PAPSS1
• NCBI Official Synonym Full Names: 3'-phosphoadenosine 5'-phosphosulfate synthase 1
• NCBI Official Symbol: PAPSS1
• NCBI Official Synonym Symbols: SK1; PAPSS; ATPSK1
• NCBI Protein Information: bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1; SK 1; PAPSS 1; PAPS synthase 1; OTTHUMP00000161864; sulfurylase kinase 1; 3-prime-phosphoadenosine 5-prime-phosphosulfate synthase 1
• UniProt Protein Name: 3'-phosphoadenosine 5'-phosphosulfate synthase 1
• Protein Family: Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase
• UniProt Gene Name: PAPSS1
• UniProt Entry Name: Q6IAX6_HUMAN

NCBI Description

Three-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) is the sulfate donor cosubstrate for all sulfotransferase (SULT) enzymes (Xu et al., 2000 [PubMed 10679223]). SULTs catalyze the sulfate conjugation of many endogenous and exogenous compounds, including drugs and other xenobiotics. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2 (MIM 603005).[supplied by OMIM]

Uniprot Description

PAPSS1: Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate- activation pathway. Also involved in the biosynthesis of sulfated L-selectin ligands in endothelial cells.

Protein type: Other Amino Acids Metabolism - selenoamino acid; EC 2.7.1.25; Kinase, other; Nucleotide Metabolism - purine; Energy Metabolism - sulfur; EC 2.7.7.4

Chromosomal Location of Human Ortholog: 4q24

Cellular Component: cytosol

Molecular Function: adenylylsulfate kinase activity; ATP binding; sulfate adenylyltransferase (ATP) activity; nucleotidyltransferase activity

Biological Process: sulfate assimilation; glycosaminoglycan metabolic process; xenobiotic metabolic process; carbohydrate metabolic process; pathogenesis; phosphorylation; skeletal development; 3'-phosphoadenosine 5'-phosphosulfate metabolic process; 3'-phosphoadenosine 5'-phosphosulfate biosynthetic process