Western Blot (WB)
(Western Blot: Sample: Recombinant PF4, Mouse)
Rabbit anti-Mouse Platelet Factor 4 (PF4) Polyclonal Antibody | anti-PF4 antibody
Polyclonal Antibody to Platelet Factor 4 (PF4)
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Sample: Recombinant PF4, Mouse)
Western Blot (WB)
(Western Blot: Sample: Recombinant PF4, Mouse)
Western Blot
(Western Blot; Sample: Human Platelet lysatePrimary Ab: 1ug/ml Rabbit Anti-Mouse PF4 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Western Blot
(Western Blot; Sample: Human Platelet lysatePrimary Ab: 1ug/ml Rabbit Anti-Mouse PF4 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Western Blot
(Western Blot; Sample: Mouse Spleen lysatePrimary Ab: 1ug/ml Rabbit Anti-Mouse PF4 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Western Blot
(Western Blot; Sample: Mouse Spleen lysatePrimary Ab: 1ug/ml Rabbit Anti-Mouse PF4 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Spleen tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Spleen tissue))
NCBI and Uniprot Product Information
Uniprot Description
PF4: Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. Belongs to the intercrine alpha (chemokine CxC) family.
Protein type: Apoptosis; Chemokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 5|5 E1
Cellular Component: cytoplasm; extracellular region; extracellular space; protein complex; vesicle
Molecular Function: chemokine activity; CXCR chemokine receptor binding; CXCR3 chemokine receptor binding; cytokine activity; heparin binding
Biological Process: chemotaxis; cytokine-mediated signaling pathway; defense response; defense response to protozoan; G-protein coupled receptor signaling pathway; immune response; inflammatory response; killing by host of symbiont cells; leukocyte chemotaxis; negative regulation of angiogenesis; negative regulation of cytolysis; negative regulation of megakaryocyte differentiation; negative regulation of MHC class II biosynthetic process; platelet activation; positive regulation of cAMP metabolic process; positive regulation of macrophage differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tumor necrosis factor production; protein complex assembly; regulation of cell proliferation; response to lipopolysaccharide
Research Articles on PF4
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Product Notes
The PF4 pf4 (Catalog #AAA2003720) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Platelet Factor 4 (PF4) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Platelet Factor 4 (PF4) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, Immunoprecipitation (IP). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the PF4 pf4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Platelet Factor 4 (PF4), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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